XPF、p53蛋白在胃癌组织中的表达及与幽门螺杆菌L型感染的关系

Relationship between Helicobacter pylori L-form infection and expression of XPF and p53 in gastric carcinoma

目的探讨XPF和p53蛋白在胃癌组织中的表达及与幽门螺杆菌L型(Helicobacter pylori L-form,Hp-L型)感染的关系。方法应用免疫组化ABC法和Giemsa染色法检测239例胃癌和癌旁正常胃黏膜组织(对照组)中XPF、p53、Hp-L型蛋白的表达及Hp-L型感染情况。结果 (1)XPF和p53蛋白在胃癌组及对照组中的表达差异均有统计学意义(P均<0.01)。XPF和p53的表达呈正相关(r=0.196,P<0.01)。XPF表达仅与Laurén分类有关(P<0.05)。p53表达仅与浸润深度有关(P<0.05)。(2)胃癌组织中Giemsa染色Hp-L型检出和Hp-L型抗原表达同时阳性的阳性率为73.22%(175/239),与对照组相比差异有统计学意义(P<0.01)。(3)胃癌组织中Hp-L型阳性组XPF和p53蛋白的阳性率高于Hp-L型阴性组(P均<0.01)。胃癌组织中Hp-L型阳性与XPF和p53的阳性表达均呈正相关(r=0.32和0.296,P均<0.01)。(4)单因素分析发现肿瘤浸润深度、远处转移、TNM分期、XPF和p53表达均与胃癌患者预后有关(P<0.05)。Cox风险模型多因素分析发现XPF是影响胃癌预后的独立因素(P<0.05),XPF阳性者具有更短的生存期。当与浸润深度及远处转移结合评估时,校正危险度增加3.791倍。结论 XPF与p53、Hp-L型在胃癌发生、发展过程中起重要作用,且XPF高表达预示预后不良。Hp-L型感染可能上调XPF和p53的表达。

Purpose To investigate the relationship between Helicobacter pylori L-form （ Hp-L） infection and expression of XPF and p53 in gastric carcinoma. Methods XPF, p53 and Hp-L were examined in 239 cases of gastric carcinoma and healthy individuals by means of Giemsa stain and immunohistochemical staining with ABC method. Results XPF and p53 expression in gastric carcinoma was significantly higher than that of the surrounding tissue （P〈0. 01）. There were positive correlation between the expression of XPF and p53 （r=0. 196, P〈0. 01）. The expression of XPF was related to the Lauren classification （P〈0. 05）. The expression of p53 was associated to the depth of invasion （P〈0. 05）. （2） 175 （73. 22%） cases were Hp-L positive by both Giemsa and immunohisto-chemical staining. Hp-L expression in gastric carcinoma were significantly higher than that of the surrounding tissue （P〈0. 01）. （3） In the Hp-L positive group, XPF and p53 were significantly higher than in that of the Hp-L negative group （P〈0. 01）. Positive corre-lation existed between positive Hp-L staining and the expression of XPF and p53 （r=0. 32, r=0. 296, P〈0. 01）. （4） Univariate a-nalysis showed that depth of invasion, distant metastasis, TNM stage, XPF and p53 were related to the prognosis of gastric carcinoma （ P〈0. 05）. Multivariate Cox model analysis showed that XPF was an independent prognostic factor for the patients （P〈0. 05）. The pa-tients with positive expression of XPF had a shorter survival time. When combined with the depth of invasion and distant metastasis, the correction risk increased by 3. 791 times. Conclusion XPF, p53 and Hp-L may play very important roles in the development of gastric carcinoma. The high expression of XPF implies poorer prognosis. Hp-L infection may be related to the up-regulated expression of XPF and p53.