食管组织中Cx43和E-cad的表达及其相关性分析

Expression and correlation of Cx43 and E-cad in esophageal carcinoma

目的观察Cx43和E-cad蛋白在食管鳞癌及正常食管组织中的表达及二者表达的相关性。方法采用免疫组化法及Western blot法检测Cx43和E-cad在食管鳞癌及正常食管组织中的表达,并分析二者表达与临床病理参数之间的关系及二者的相关性。结果免疫组化和Western blot结果显示,Cx43和E-cad在食管鳞癌组织中的表达均显著低于正常食管组织(P<0.05);Cx43表达与食管鳞癌淋巴结转移有关,E-cad的表达与食管鳞癌呈组织分化程度有关。Cx43与E-cad在同一例食管鳞癌组织中的表达呈显著正相关。结论 Cx43与E-cad蛋白在食管鳞癌组织中均低表达,联合检测Cx43和E-cad基因表达可能对食管鳞癌的生物学行为和预后评估起一定作用。

Purpose To investigate the expression of the Cx43 and E-cad protein in esophageal carcinoma and normal esophageal tis-sue and to discuss their relationship. Methods SP immunohistochemical and Western blot analysis were used to detect the expression of Cx43 and E-cad in esophageal carcinoma and normal esophageal tissue. The relation between their expression and esophageal carci-noma was investigated. Results Immunohistochemical assay and Western blot analysis showed the expression levels of Cx43 and E-cad in esophageal carcinoma were significantly lower than that in normal esophageal tissue. The expression of Cx43 had correlation with lymph node metastasis. The expression of E-cad had correlation with degrees of pathological differentiation. There was a positive rela-tionship between the expression of Cx43 and E-cad in same sample. Conclusion Expression of Cx43 and E-cad protein is low in e-sophageal carcinoma, which can be helpful in the prediction of the biological behavior and prognosis of esophageal carcinoma.