肾透明细胞癌中EphA1蛋白的表达及临床意义

Expression of Eph A1 protein in clear cell renal cell carcinomas and its clinical significance

目的探讨受体酪氨酸激酶Eph A1在肾细胞癌(renal cell carcinomas,RCC)中的表达,并分析其表达与临床病理特征的关系。方法采用免疫组化法检测144例肾透明细胞癌、18例肾嫌色细胞癌和6例肾乳头状癌组织中Eph A1蛋白的表达,并复习相关文献。结果 Eph A1在正常肾小管中高表达。Eph A1蛋白在64.6%(93/144)肾透明细胞癌中呈阴性或弱阳性,35.4%(51/144)呈阳性。Eph A1蛋白在肾嫌色细胞癌和乳头状癌中均呈阳性。Eph A1蛋白高表达与肾透明细胞癌患者年龄(P<0.001)、性别(P=0.016)以及核级(P<0.001)有关,与肿瘤直径无关(P=0.316)。结论 Eph A1蛋白有可能成为肾透明细胞癌临床预后的新标志物。

Purpose To investigate the expression of EphA1 in renal cell carcinomas （ RCC） and to analyze its correlation with clini-copathological parameters in order to explore the clinical significance of EphA1 protein in renal cell carcinomas （ RCC） . Methods The immunocytochemical method was employed to measure the expression of EphA1 in 144 clear cell RCC （ ccRCC） tissues, 18 chro-mophobe RCC tissues and 6 papillary RCC tissues. Correlation between EphA1 protein expression and clinical parameters was evaluated by statistics. Results High level of the expression of EphA1 was observed in all normal renal tubes. The EphA1 protein was negative-ly or weakly expressed in 93 out of 144 ccRCC （64. 6%） and positively expressed in 51 out of 144 ccRCC （35. 4%）. Positive expres-sion of EphA1 was observed in all samples of chromophobe RCC and papillary RCC. The high level expression of the EphA1 protein was significantly associated with younger patients （P〈0. 001）, sex （P=0. 016） and lower nuclear grade （P〈0. 001）. No significant relation between the expression of EphA1 and tumor diameter was found （ P=0. 316 ） . Conclusion EphA1 protein may be a new marker for the prognosis of ccRCC.