肿瘤转移抑制基因KAI1/CD82及细胞分子CD44v6在人喉鳞癌中的表达及意义

Expression of KAI1 / CD82 and CD44v6 in human laryngeal squamous cell carcinoma and their clinical significance

目的探讨肿瘤转移抑制基因KAI1/CD82和细胞分子CD44v6在人喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)中的表达及意义,并探讨二者之间的关系。方法采用免疫组化En Vision法检测64例LSCC和15例正常喉黏膜(normal laryngeal mucosa,NLM)组织中KAI1/CD82和CD44v6的表达。结果 LSCC组织中KAI1/CD82蛋白的阳性率(37.50%)显著低于NLM组织(86.67%)(P=0.031);LSCC组织中CD44v6蛋白的阳性率(75.00%)显著高于NLM组织(26.67%)(P=0.011)。KAI1/CD82表达与LSCC临床分期、分化程度及淋巴结转移相关(P<0.05),与预后无关(P>0.05);CD44v6表达与LSCC分化程度、淋巴结转移及预后相关(P<0.05),与临床分期无关(P>0.05);二者均与患者年龄、性别无关(P>0.05)。KAI1/CD82与CD44v6在LSCC组织中的表达呈负相关(rs=-0.504,P=0.036)。结论 KAI1/CD82与CD44v6在LSCC的发生、发展和侵袭转移中起着相互抑制的作用,检测KAI1/CD82及CD44v6蛋白表达对判断LSCC的生物学行为具有重要价值。

Purpose To study the expression of metastasis suppressor gene （KAI1/CD82） and cell adhesion molecules（CD44v6） in human laryngeal squamous cell carcinoma （ LSCC） and its clinical significance, and to investigate their relationship. Methods EnVi-sion immunohistochemistry was used to detect the expression of KAI1/CD82 and CD44v6 protein in 64 cases of LSCC tissues and 15 ca-ses of normal laryngeal mucosa （ NLM） tissues. Results The positive rate of KAI1/CD82 in LSCC tissues （ 37. 50%） was signifi-cantly lower than that of the NLM tissues（86. 67%） （P=0. 031）, and the positive rate of CD44v6 in LSCC tissues（75. 00%） was significantly higher than that of the NLM tissues（26. 67%） （P=0. 011）. The expression of KAI1/CD82 was associated with clinical stages, grade of tumor differentiation, neck lymph node metastasis （ P〈0. 05 ） while not related with age, sex and prognosis （ P〉0. 05）. And CD44v6 with grade of tumor differentiation, neck lymph node metastasis and prognosis （P〈0. 05）, but not with age, sex and clinical stages （P〉0. 05）. In addition, KAI1/CD82 expression was negatively correlated with CD44v6 expression （rs = -0. 504, P=0. 036）. Conclusion KAI1/CD82 and CD44v6 are mutually inhibited in the tumorigenesis, progress, invasion and metastasis, and detection of the expression of KAI1/CD82 and CD44v6 may be helpful for judging the biological behaviors of LSCC.