黄芪甲苷对肿瘤坏死因子-α诱导的大鼠血管平滑肌细胞增殖、迁移的影响

Effects of Astragaloside IV on TNF-α-induced cell proliferation and migration in rat vascular smooth muscle cell

目的:研究黄芪甲苷(AS-IV)对肿瘤坏死因子(TNF-α)诱导的大鼠胸主动脉平滑肌细胞(A7r5细胞)增殖、迁移的影响。方法:建立体外TNF-α诱导的A7r5细胞增殖、迁移模型,用不同浓度AS-IV进行干预。应用WST-8法检测细胞增殖活性,用划痕实验和Transwell侵袭实验分别检测细胞迁移、侵袭能力。结果:A7r5细胞经TNF-α作用后,其增殖活性、迁移距离、侵袭能力均明显增加,与对照组相比有显著性差异(P<0.05);而经AS-IV干预后,细胞增殖活性降低、迁移距离变短、侵袭能力下降,与TNF-α组相比有显著性差异(P<0.05),并且这种药物抑制作用呈时间与剂量依赖性。结论:AS-IV可抑制TNF-α诱导的血管平滑肌细胞增殖、迁移,这可能是AS-IV预防和治疗血管再狭窄、动脉粥样硬化等血管增生性疾病的作用机制之一。

Objective：To investigate the effects of Astragaloside IV（AS-IV）on proliferation and migration of rat vascular smooth muscle cells from thoracic aorta（A7r5cells）that were induced by tumor necrosis factor-α（TNF-α）.Method：The model of A7r5 cells proliferation and migration was established by TNF-αinducer in vitro,and intervened by different concentrations of AS-IV.A WST-8assay was used to evaluate cell proliferation activity.Wound migration assay and Transwell invasion assay were used to analyze cell migratory and invasive capability.Result：The proliferative activity,migratory distance and invasive capacity of A7r5 cells were obviously increased in the TNF-αgroup.There was a significant difference as compared with the control group（P〈0.05）.But when pretreated with AS-IV ahead of time,the proliferative activity,migratory distance and invasive capacity of A7r5 cells were decreased in a time-and dose-dependent manner.There was a significant difference as compared with the TNF-αgroup（P〈0.05）.Conclusion：AS-IV exerts inhibitory effects on vascular smooth muscle cells proliferation and migration,which may be one of the mechanisms that AS-IV can prevent and treat the vascular proliferative diseases such as reangiostenosis and atherosclerosis.