牦牛和犏牛DDX25/GRTH基因序列及其在睾丸组织的表达水平

Sequencing and Expression of DDX25/GRTH mRNA in yak and Cattle-yak Testis

为了解DDX25基因的结构、功能及对犏牛雄性不育的影响。采用RT-PCR技术克隆获得牦牛和犏牛的DDX25基因,对核苷酸序列和氨基酸序列进行生物信息学分析,运用实时荧光定量PCR技术检测睾丸组织DDX25 mRNA的表达情况。结果表明,牦牛和犏牛DDX25基因编码区序列全长均为1 452bp,编码483个氨基酸,氨基酸存在19个磷酸化位点,无信号肽。牦牛睾丸组织DDX25基因表达水平显著高于犏牛,DDX25基因在犏牛睾丸组织的低表达与其雄性不育存在一定关系,该基因可作为研究犏牛雄性不育的候选基因。

To understand the structure of DDX25,gene function and its impact on cattle-yak male sterility,DDX25 gene was cloned by RT-PCR from cattle-yak and yak,conducting bioinformatics analysis based on the sequences of nucleotide and amino acid,and DDX25 mRNA expression in testis was detected by Real-time PCR.The results showed that DDX25 gene coding sequences in yak and cattleyak is the full length of 1 452 bp,encoding 483 amino acids with 19 phosphorylation sites,without signal peptide.DDX25 gene expression level in testis of yak were significantly higher than in testis of cattle-yak.Low expression of DDX25 gene in testis of cattle-yak has a certain relationship with their male sterility,so the gene can be used as a candidate gene for male sterility cattle-yak.