有序性三维胶原支架材料的制备与MSCs诱导效应研究

Fabrication of 3D Collagen Scaffolds with Orientated Channels and MSCs Inductive Effect

目的探讨有序支架材料联合骨髓间充质干细胞(mesenchymal stem cells,MSCs)修复脊髓损伤的可行性。方法通过温度梯度法制备有序性胶原支架材料,以神经元轴突延伸的方向性验证,并诱导材料上MSCs分化成神经元。结果扫描电子显微镜下发现,胶原支架材料方向性成孔,孔径大小为(60±10)μm,能诱导脊髓背根神经节(dorsal root ganglion,DRG)中神经元方向性延伸轴突。有序性材料上的MSCs沿孔径的方向排列细胞体,诱导分化的神经元也具有方向性。结论有序性胶原支架材料能诱导MSCs和MSCs源神经元方向性延伸轴突,为脊髓损伤治疗提供一种可行性方案。

Objective To investigate the feasibility of combining uniaxial guidance collagen scaffolds and bone marrew mesenchymal stem cells（ MSCs） for the repair of spinal cord injury. Methods Temperature gradients technique was employed to fabricate collagen scaffold containing highly orientated channels in this study. Neurons from MSCs were also induced in scaffolds. Results Scanning electron microscopy（ SEM） analysis revealed that scaffolds with axially directed pore architectures were obtained and the pore size was（ 60 ± 10）μm. Axons extending from the cell bodies of neurons in spinal cord dorsal root ganglion（ DRG） were guided by scaffold with direction. Induced neuronal cell from MSCs also displayed an orientated morphology and extended axons along the direction of microchannels. Conclusion The results lay the feasibility foundation of combining MSCs and orderly collagen scaffolds for treatment of spinal cord injury.