体重指数影响骨钙素基因HindⅢ多态性与中国绝经前妇女空腹血糖间的关联

Association between HindⅢ RFLP of the osteocalcin gene and fast plasma glucose level in premenopausal Chinese women:can be modified by body mass index

目的:考察骨钙素(BGP)Hind III基因多态性是否与中国绝经前妇女空腹血糖(FPG)变异间存在关联;检测BGP基因型和体重指数(BMI)间的关联是否可以部分地解释BGP基因型与FPG间的关联。方法:研究对象是328名中国绝经前妇女,年龄21岁以上[(33.2±5.9)岁],FPG值平均为4.92 mmol/L(标准差,0.81),采用聚合酶链反应(PCR)-限制性片段长度多态(RFLP)法对所有研究对象的BGP Hind III位点进行基因分型。结果:BGP Hind III多态性与校正年龄的FPG以及未用年龄校正的FPG间均存在显著的关联(P值分别为0.028和0.041),且BGP基因可以解释3.32%的FPG变异。但BGP Hind III多态性与年龄和BMI校正的FPG间无显著关联(P=0.517)。此外,BGP Hind III多态性与未校正的BMI(P=0.002)或年龄校正的BMI(P=0.003)都有显著的关联,且BMI与未校正的FPG也存在显著相关(r=0.424,P<0.01),BMI可以解释20.15%的FPG变异。结论:BMI可影响BGP Hind III多态性与中国绝经前妇女FPG间的关联。该研究结果提示协变量如BMI在FPG的遗传关联研究中的重要性。

AIM： To investigate whether osteocalcin（ BGP） Hind III genetic polymorphism is associated with fasting plasma glucose（ FPG） variation in premenopausal Chinese women; and to test the hypothesis that the association between BGP genotypes and FPG may be partially accounted for by an association between BGP genotypes and BMI.METHODS： The study subjects were 328 unrelated premenopausal Chinese women aged 21 years and over [（ 33. 2 ± 5. 9） years],with an average FPG of4. 92 mmol / L（ SD,0. 81）. All subjects were genotyped at the BGP Hind III loci using polymerase chain reaction（ PCR）-restriction fragment length polymorphism（ RFLP）. RESULTS： Raw FPG was significantly associated with BGP Hind III polymorphism with and without adjusting for age（ P = 0. 028 and 0. 041,respectively）. This genetic effect can explain 3. 22% of FPG variation. However,no significant association was found between BGP Hind III polymorphism and FPG adjusted by both age and body mass index（ BMI）（ P = 0. 517）. In addition,we observed a significant association between BGP Hind III polymorphism with unadjusted BMI（ P =0. 002） or BMI adjusted for age（ P = 0. 003）. The raw FPG was also found to be significantly correlated with BMI（ r = 0. 424; P〈 0. 01）,with BMI explaining 20. 15% of the variation of FPG. CONCLUSION： BMI may modify the association between the Hind III polymorphism of the BGP gene and FPG in premenopausal Chinese women,which highlights the importance of incorporating covariates such as BMI into genetic association studies of FPG.