健康妇女妊娠不同阶段阴道乳酸杆菌分布状况研究

The characteristics of vaginal lactobacilli during different pregnancy

目的探讨妊娠不同阶段阴道乳酸杆菌的分布特点。方法选择120例健康妊娠妇女,取其阴道分泌物行乳酸杆菌菌种鉴定,并对乳酸杆菌产生过氧化氢的情况进行检测。比较不同妊娠期阴道乳酸杆菌菌种的分布及不同乳酸杆菌产生过氧化氢的能力。结果 86.7%的妊娠期妇女阴道内能检测出乳酸杆菌。嗜酸乳酸杆菌为优势菌(76.9%),其次为德氏乳酸杆菌亚种(15.4%),卷曲乳酸杆菌最少(7.7%)。妊娠期阴道内能产生过氧化氢的乳酸杆菌的优势菌种为嗜酸乳酸杆菌。结论妊娠期阴道内能产生过氧化氢的乳酸杆菌的优势菌种为嗜酸乳酸杆菌,在维持妊娠期妇女阴道微生态平衡、防御阴道感染中起着重要作用。

Objective To investigate the characteristics of distribution of vaginal lactobacilli during pregnancy. Methods A total of 120 healthy pregnant women were selected as sample. The identification of lactobacillus strains in vaginal secretions were measured, and the production of hydrogen peroxide by lactobacillus were detected. Vaginal lactobacillus distribution and ability of producing hydrogen peroxide by different lactobacillus species during different gesta- tion period were compared. Results Lactobacillus genus was presented in 86.7% of pregnant women studied. L. acidophilus was the predominant （76.9%）, followed by L. delbrueckii ssp delbrueckii （15.4%）, and L. crispatus （7.7%）. L. acidophilus was the predominant hydrogen peroxide positive Lactobacilli species colonizing in the vagina of pregnant women. Conclusion L. acidophilus is the predominant Lactobacilli species colonizing in the vagina of healthy pregnant women, they play an important role in the ecological balance maintenance of vaginal microflora and the prevention from vaginal infections in pregnant women.