胃癌组织中I2PP2A基因表达量及基因沉默对胃癌细胞恶性生物学的影响

Expression of I2PP2A gene in gastric cancer tissue and the effect of gene silencing on malignant biology of gastric cancer cells

目的:研究胃癌组织中蛋白磷酸酶2A抑制剂-2(I2PP2A)基因表达量及基因沉默对胃癌细胞恶性生物学的影响。方法:采集胃癌患者的胃癌组织及癌旁正常组织,培养胃癌细胞株BGC-823并转染I2PP2A的siRNA,检测组织标本中I2PP2A的mRNA含量和蛋白含量;检测细胞样本中的凋亡细胞比例、不同细胞周期比例、Cyclin B2、Cyclin D1、CDK1、Bcl-2、Survivin的蛋白含量。结果:胃癌组织中I2PP2A的mRNA含量和蛋白含量均高于癌旁正常组织;转染I2PP2A-siRNA后,胃癌细胞的G0/G1期细胞比例、凋亡细胞比例高于阴性对照组,S期细胞和G2/M期细胞比例低于阴性对照组,Cyclin B2、Cyclin D1、CDK1、Bcl-2、Survivin的蛋白含量均低于阴性对照组。结论:胃癌组织中I2PP2A基因的表达量异常增多;沉默I2PP2A基因能够通过抑制Cyclin B2、Cyclin D1、CDK1、Bcl-2、Survivin的表达来诱导胃癌细胞凋亡并使细胞周期停滞于G0/G1期。

Objective：To study the expression of I2PP2 Agene in gastric cancer tissues and the effects of gene silencing on malignant biology of gastric cancer cells.Methods：Gastric cancer tissues and adjacent normal tissue in patients with gastric cancer were collected,gastric cancer cell lines BGC-823 were cultured and I2PP2 AsiRNA was transfected.The mRNA contents and protein contents of I2PP2 Ain tissue samples were detected;the proportions of apoptotic cells and different cell cycles as well as protein contents of Cyclin B2,Cyclin D1,CDK1,Bcl-2and Survivin in cell samples were detected.Results：mRNA contents and protein contents of I2PP2 Ain gastric cancer tissues were higher than those in adjacent normal tissues.After transfection of I2PP2A-siRNA,the proportions of G0/G1 phase cells and apoptotic cells in gastric cancer cells were higher than those in negative control group,proportions of S-phase cells and G2/M phase cells were lower than those in negative control group,and protein contents of Cyclin B2,Cyclin D1,CDK1,Bcl-2and Survivin were lower than those in negative control group.Conclusion：The expression of I2PP2 Ain gastric cancer tissue abnormally increases;silencing I2PP2 Agene can inhibit the expression of Cyclin B2,Cyclin D1,CDK1,Bcl-2and Survivin to induce gastric cancer cell apoptosis and make cell cycle stagnate in G0/G1 phase.