前列腺癌骨转移患者血清ODF、OCIF含量及临床价值评估

Detection of serum ODF and OCIF contents in prostate cancer patients with bone metastasis and its clinical value assessment

目的:研究前列腺癌骨转移患者血清ODF、OCIF含量及其临床价值。方法:采集前列腺癌骨转移骨转移和非骨转移患者的血清,检测ODF、OCIF含量;培养前列腺癌DU145细胞,用不同浓度的ODF、OCIF处理后,检测细胞迁移率以及LASS-2/TMSG-1的mRNA含量。结果:骨转移组血清中ODF、OCIF的含量以及ODF/OCIF的比值均高于非骨转移组(P<0.05);骨转移病灶数目越多,血清ODF含量越高、而OCIF含量越低;ODF处理能够剂量依赖性地增加前列腺癌细胞迁移率、减少LASS-2/TMSG-1的mRNA含量;OCIF处理能够剂量依赖性地降低前列腺癌细胞迁移率、增加LASS-2/TMSG-1的mRNA含量。结论:前列腺癌骨转移患者血清中ODF、OCIF含量均升高,且以ODF升高的效应更为明显;ODF具有促进前列腺癌细胞迁移的作用、而OCIF具有抑制作用。

Objective：To detect serum ODF and OCIF contents in prostate cancer patients with bone metastasis and its clinical value.Methods：Serum of prostate cancer patients with and without bone metastasis was collected,then ODF and OCIF contents were detected;prostate cancer DU145 cells were cultured and treated with different concentrations of ODF and OCIF,then cell migration rates as well as mRNA contents of LASS-2/TMSG-1were detected.Results：Serum ODF and OCIF contents as well as ODF/OCIF ratio of bone metastasis group were all higher than those of non-bone metastasis group;the more the number of metastatic bone lesions was,the higher the serum ODF content was,and the lower the OCIF content was;ODF treatment could increase prostate cancer cell migration rates and decrease mRNA contents of LASS-2/TMSG-1on dose-dependent manner;OCIF treatment could decrease prostate cancer cell migration rates and increase mRNA contents of LASS-2/TMSG-1on dose-dependent manner.Conclusion：Serum ODF and OCIF contents in prostate cancer patients with bone metastasis increase,and ODF increase effect is more obvious;ODF has promoting effect on prostate cancer cell migration while OCIF has inhibiting effect.