摘要
为了弄清贵州主要蔗区甘蔗花叶病害发生情况,本研究通过PCR检测方法,对采自贵州主要蔗区的52份疑似花叶病样品进行检测,结果表明：39份样品呈阳性（检出率为75%）。对阳性样品的PCR扩增产物测序比对,PCR扩增产物的基因序列与Gen Bank中SrMV分离物的基因序列同源性在96%~99%,由此证实贵州主要蔗区甘蔗普遍感染SrMV。
The study aims to find out mosaic disease epidemic situation in main sugarcane field in Guizhou. Inthis research, 52 sugarcane leaf samples with disease symptom from the main sugarcane field in Guizhou weredetected by PCR. The results showed that 39 sugarcane samples were positive（75% samples were infectedwith SrMV）. By sequencing the PCR amplified products of positive samples, the sequence homology of the PCRamplified product with SrMV isolates genes in Gen Bank was 96%-99%, thus confirmed the widespreadinfection of SrMV in main sugarcane field in Guizhou.
出处
《中国农学通报》
2015年第30期231-233,共3页
Chinese Agricultural Science Bulletin
基金
国家星火计划项目"旱坡地甘蔗高效栽培关键技术示范与推广"(2014GA820009)
贵州省农业攻关项目"强宿根抗旱甘蔗新品种的培育及应用"(黔科合NY字[2012]3034号)
贵州省农科院专项"黔蔗吨糖田关键技术研究与示范"(黔农科院院专项[2012]003号)
贵州省农科院创新基金"CO2气肥在开放组培中的创新应用研究"[黔农科合创新基金项目(2011012号)]
