摘要
通过紫外-可见吸收光谱、瞬态荧光光谱以及圆二色谱来比较光疗药物竹红菌甲素及铂竹红菌甲素复合物与牛血红蛋白的相互作用.结果表明,虽然竹红菌甲素及铂竹红菌甲素复合物与牛血红蛋白的反应机理都是基于电子转移的静态猝灭,但是铂竹红菌甲素复合物与牛血红蛋白的反应不仅要比竹红菌甲素强,而且反应后牛血红蛋白的微环境和构像的改变也要大于竹红菌甲素.
The interaction mechanisms of Hypocrellin A（HA）and its platinum complex（Pt-HA）with bovine hemoglobin（BHb）were compared by ultraviolet-visible（UV-Vis）absorption,time-resolved fluorescence spectra,circular dichroism（CD）and induced circular dichroism（ICD）spectra,respectively. The UV-Vis results revealed that both HA and Pt-HA could interact with BHb and the latter interaction was stronger than HA. In addition,quenching mechanism of HA and Pt-HA were both static process,which were ascribed to the electron transfer. Moreover,CD and ICD spectrum revealed that the microenvironment and conformation of BHb were changed after conjugation with HA and Pt-HA,as the latter altered more acute. Finally,the comparison results demonstrated that the interaction of Pt-HA and BHb was stronger and more stable than HA.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
北大核心
2015年第3期14-24,共11页
Journal of Nanjing Normal University(Natural Science Edition)
基金
Project of Department of Science and Technology of Jiangsu Province(BK20131394
BZ201210)
