加拿大一枝黄花花序中黄酮类化合物的抑菌及保鲜作用研究

Antimicrobial Activity and Preservation Function of the Flavonoids Extracted from Inflorescence of Solidago canadensis L.

提取加拿大一枝黄花(Solidago canadensis L.)花序中的黄酮类物质,并研究其抑菌及保鲜作用.利用有机溶剂乙醇提取加拿大一枝黄花花序中的黄酮类物质;采用抑菌圈法进行抑菌性研究;采用测定好果率的方法研究其保鲜作用.当提取的加拿大一枝黄花花序中的黄酮类化合物的浓度为0.056 mg/m L时,即显示对杆菌的抑制作用,其对真菌的抑制作用较弱,对各菌种的抑制效果为:大肠杆菌(Escherichia coli)>枯草芽孢杆菌(Bacillus subtilis)>酿酒酵母(Saccharomyces cerevisiae),对黑曲霉(Aspergillus niger)没有抑制作用.喷洒浓度为0.028 mg/m L的黄酮提取液的樱桃番茄实验组较对照组能有效降低霉变率,保鲜作用显著提高.加拿大一枝黄花花序中的黄酮类化合物具有一定的的抑菌及保鲜作用.该研究为加拿大一枝黄花的综合利用提供参考.

The aim was to extract the flavonoids from inflorescence of Solidago canadensis L. and study their antimicrobial activity and preservation function. The flavonoids were optimally extracted from inflorescence of Solidago canadensis L.with ethanol. The antimicrobial activity of the flavonoids was tested by the experiments of anti-bacterium-circles. The good fruit rate in cherry tomatoes was tested to study the preservation function of the flavonoids. When the concentration of concentrated flavonoids extracted from inflorescence of Solidago canadensis L. reached 0.056 mg/m L,it could be judged that inflorescence extracts inhibition activity were：Escherichia coliBacillus subtilisSaccharomyces cerevisiae.Besides,the total flavonoids presented no inhibition to Aspergillus niger. When sprayed with 0.028 mg/m L flavonoides solution,the cherry tomato of experimental group could effectively reduce the rates of its mildewing with another group sprayed with distilled water as a control. The total flavonoids in inflorescence of Solidago canadensis L. exhibited some antimicrobial activity and preservation function on cherry tomato,which would provide valuable information for the development of Solidago canadensis L.