变应性鼻炎和支气管哮喘患者CD4^+CD25^+调节性T细胞与总IgE表达的比较及其相关性研究

Comparative and correlated analysis of the expression of CD4^+CD25^+ Regulatory T cells and IgE in Allergic Rhinitis and Bronchial Asthma

目的通过比较变应性鼻炎和哮喘患者总Ig E(t Ig E)和CD4+CD25+调节性T细胞的表达,并分析CD4+CD25+调节性T细胞与t Ig E的相关性,旨在进一步探讨变应性鼻炎与哮喘的发病机制,以及二者之间的联系与区别,探索哮喘的早期防治及减少其发生率的新途径。方法分别用流式细胞术和免疫印迹技术检测变应性鼻炎、哮喘患者及正常对照组外周血CD4+CD25+调节性T细胞的百分率和血清t Ig E水平。结果变应性鼻炎及哮喘患者外周血CD4+CD25+调节性T细胞明显低于正常对照组,变应性鼻炎组低于哮喘组;变应性鼻炎及哮喘患者血清中的t Ig E水平明显高于正常对照组,哮喘组高于变应性鼻炎组;CD4+CD25+调节性T细胞与t Ig E呈显著负相关。结论 CD4+CD25+调节性T细胞及t Ig E的水平和病情轻重相关,诱导调节性T细胞的表达可望成为治疗变应性疾病的一个新途径。

Objective Comparing the expression of total Ig E（t Ig E） and CD4＋CD25＋ Regulatory T cells（CD4＋CD25＋Tr） in patients with Allergic Rhinitis（AR） and Bronchial Asthma（BA）, analyzing the correlation between CD4＋CD25＋Tr and t Ig E in order to further investigate the pathogenesy of AR and BA, and the relation and difference of them, explore a new way for the early prevention and treatment of asthma to reduce its incidence. Methods The percentage of CD4＋CD25＋Tr in peripheral blood and the level of t Ig E in serum of patients with AR、BA and 20 cases healthy people were detectded by flow cytometry and western blot. The relathion between CD4＋CD25＋Tr and t Ig E was analyzied. Results The percentage of CD4＋CD25＋ Tr in patients with AR and BA were significantly lower than that in normal controls, the level of t Ig E in patients with AR and BA were significantly higher than that in normal controls,and that in BA was higher than in AR.The level of t Ig E was increased obviously when the patients＇ condition became more serious. Conclusion The level of CD4＋CD25＋Tr and t Ig E correlated with the degree of clinical condition.Effective treatment on AR was the key to control BA.