五味子甲素、五味子乙素在小鼠体内的组织分布研究

Distribution characteristics of schisandrin A and schisandrin B in mouse tissues

目的:研究五味子甲素、五味子乙素在小鼠体内的组织分布特点。方法:按25 mg·kg-1剂量五味子甲素和五味子乙素分别1次灌胃给药后0.5、1、2、3、4 h,采集组织样品并进行处理。采用高效液相色谱法对五味子甲素和五味子乙素在小鼠心、肝、肾、脑中的浓度进行测定;色谱条件:采用Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-水(70∶30)为流动相,流速1.0 m L·min-1,检测波长254 nm,柱温40℃,进样量:20μL。结果:五味子甲素和五味子乙素在小鼠体内心脏、肝脏、肾脏、脑组织中均有分布。给药1 h后五味子甲素和五味子乙素在心脏、脑组织中浓度达到峰值,心脏中分别为(6.65±0.48)μg·m L-1和(7.68±0.76)μg·m L-1,脑中分布为(7.50±0.69)μg·m L-1和(21.41±2.54)μg·m L-1;给药2 h后五味子甲素和五味子乙素在肝脏中浓度达到峰值,分别为(15.66±1.69)μg·m L-1和(12.24±0.65)μg·m L-1;给药3 h后五味子甲素和五味子乙素在肾脏中浓度达到峰值,分别为(7.98±0.62)μg·m L-1和(7.39±0.19)μg·m L-1。整个分布情况显示,各时间点五味子甲素在肝脏中浓度均高于其他组织,且达峰时浓度明显高于心脏、肾脏、脑,其次是肾脏,浓度较低的为心脏和脑。各时间点五味子乙素在脑中浓度均高于其他组织,且达峰时浓度明显高于肝脏、心脏、肾脏;其次是肝脏,浓度较低的为肾脏和心脏。结论:灌胃给药后五味子甲素的峰值和各时间点的浓度在肝脏中最高;五味子乙素的峰值和各时间点的浓度在脑中最高。

Objective： To study the distribution characteristics of schisandrin A and schisandrin B in mouse tissues.Methods： 25 mg·kg- 1schisandrin A and schisandrin B were given to mice intragastrically respectively. The mice were randomly sacrificed at 0. 5,1,2,3,4 h after the intragastric administration,and the heart,liver,kidney and brain tissue samples were collected. Schisandrin A and schisandrin B concentrations in various tissues were determined by HPLC using an Agilent C18column（ 250 mm × 4. 6 mm,5 μm） with the mobile phase of methanol- water（ 70∶ 30） at a flow rate of 1. 0 m L·min- 1. The detection wavelength was 254 nm,the column temperature was40 ℃,and injection volume was 20 μL. Results： Both schisandrin A and schisandrin B were distributed in mice heart,liver,kidney and brain tissues after the intragastric administration. 1 h after the administration,schisandrin A and schisandrin B concentrations in heart and brain tissues reached their peak values,（ 6. 65 ± 0. 48） μg·m L- 1and（ 7. 68 ± 0. 76） μg·m L- 1in the heart tissue,and（ 7. 50 ± 0. 69） μg·m L- 1and（ 21. 41 ± 2. 54） μg·m L- 1in the brain tissue,respectively; at 2 h,schisandrin A and schisandrin B concentrations in the liver tissue reached their peak values,（ 15. 66 ± 1. 69） μg·m L- 1and（ 12. 24 ± 0. 65） μg·m L- 1,respectively; at 3 h,schisandrin A and schisandrin B concentrations in the kidney tissue reached their peak values,（ 7. 98 ± 0. 62） μg·m L- 1and（ 7. 39 ±0. 19） μg·m L- 1,respectively. The distribution results showed that schisandrin A concentration in the liver was higher than those in the other tissues at all different time points,and the peak concentration in liver was significantly higher than that in the heart,kidney and brain; the concentration in the kidney was secondary,and the concentrations in the heart and brain were much lower. Schisandrin B concentrations in the brain at the different time points were higher than those in the other tissues at all time points,and the peak concentration was significantly higher than that in the liver,heart and kidney; the concentration in the liver was secondary,and the concentration in the kidney and heart was much lower. Conclusion： After the intragastric administration,the highest concentration of schisandrin A was observed in the liver,and the highest concentration of schisandrin B was found in the brain.