HPLC-DAD同时测定妇炎丸中5种活性成分的含量

Simultaneous determination of five kinds of components in Fuyan pills by HPLC-DAD

目的：建立高效液相色谱法同时测定妇炎丸中绿原酸、栀子苷、芍药苷、橙皮苷和连翘苷5种活性成分含量的方法。方法：采用CNW Athena C18（4.6 mm×250 mm,5μm）色谱柱,以乙腈（A）-0.2%磷酸水溶液（B）为流动相,梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长：217 nm（绿原酸、橙皮苷和连翘苷）、230 nm（栀子苷和芍药苷）。结果：绿原酸、栀子苷、芍药苷、橙皮苷和连翘苷的线性范围分别为8.80～220μg·m L-1（r=0.999 8）、4.28～107μg·m L-1（r=0.999 9）、4.16～104μg·m L-1（r=0.999 9）、8.08～202μg·m L-1（r=0.999 7）和4.52～113μg·m L-1（r=0.999 8）;平均加样回收率（n=6）分别为98.9%、99.1%、98.9%、99.3%和99.2%,RSD分别为1.0%、0.9%、1.1%、1.0%和0.9%;含量分别为0.88、0.41、0.35、0.98和0.38 mg·g-1。结论：本法专属性强,结果准确,重复性好,可用于妇炎丸的质量控制。

Objective： To establish an HPLC method for determination of five bioactive components in Fuyan pills,including chlorogenic acid,geniposide,paeoniflorin,hesperidin and phillyrin. Methods： A stable HPLC method was established and the chromatography was accomplished on a CNW Athena C18（ 4. 6 mm ×250 mm,5 μm） column. The mobile phase consisted of acetonitrile（ A）-0. 2% phosphoric acid（ B） in a gradient mode at the flow rate of 1. 0 m L·min- 1and the column temperature was set at 30 ℃. The detection wavelength was set at 217 nm for chlorogenic acid,hesperidin and phillyrin,230 nm for geniposide and paeoniflorin,respectively. Results： The linear ranges of chlorogenic acid,geniposide,paeoniflorin,hesperidin and phillyrin were 8. 80-220 μg·m L- 1（ r =0. 999 8）,4. 28-107 μg·m L- 1（ r =0. 999 9）,4. 16-104 μg·m L- 1（ r =0. 999 9）,8. 08-202 μg·m L- 1（ r =0. 999 7） and 4. 52-113 μg·m L- 1（ r =0. 999 8）,respectively. The average recoveries were 98. 9%,99. 1%,98. 9%,99. 3% and 99. 2%; and RSDs were 1. 0%,0. 9%,1. 1%,1. 0% and 0. 9%; the contents were 0. 88,0. 41,0. 35,0. 98 and 0. 38 mg·g- 1. Conclusion： The method is special,accurate and reproducible,which can be used for the quality control of Fuyan pills.