1,25-二羟维生素D_3对免疫性血小板减少症模型小鼠的治疗作用

The therapeutic effect of 1,25-dihydroxyvitamin D_3 on ITP mice model

目的观察并分析1,25-二羟维生素D3对免疫性血小板减少症(ITP)模型小鼠的治疗作用。方法将44只BALB/c小鼠分为正常对照组、模型组和1,25-二羟维生素D3治疗组。模型组经腹腔给予豚鼠抗小鼠血小板血清(GPAPS),治疗组在模型组的基础上经尾静脉注射给予100 ng/d的1,25-二羟维生素D3,正常对照组均给予相同体积的生理盐水经腹腔及尾静脉注射给药。在每次给药后24 h记录体重并采血,测量血小板计数,评价出血倾向。2周后处死小鼠并解剖,取骨髓涂片,镜下计数巨核细胞。从多方面评估1,25-二羟维生素D3对模型小鼠的治疗作用。结果注射第10天开始,1,25-二羟维生素D3治疗组小鼠进食量开始明显增加,精神和反应逐渐恢复;体重、血小板数较模型组明显上升(P<0.05);出血倾向较模型组明显降低(P<0.05);产板巨核细胞数较模型组高,低于正常对照组,而巨核细胞数较模型组低,高于正常对照组,差异有统计学意义。结论 1,25-二羟维生素D3对模型小鼠在一般情况的改善、体重及血小板数的上升、出血倾向的降低和促进骨髓血小板生成等方面表现出一定影响。

Objective To observe and analyze the therapeutic effect of 1,25-dihydroxyvitamin D3 on the immune thrombocytopenia（ ITP） model in mice. Methods 44 BALB / c mice were divided into three groups： normal control group,model group and 1,25-dihydroxyvitamin D3 treatment group. The model group was given the GP-APS intraperitoneally,while the treatment group,on the basis of the model group,was administered 100 ng / d of 1,25-dihydroxyvitamin D3 by intravenous injection,and the normal control group was given the same volume of saline through both intraperitoneal and intravenous injection. The mice were taken blood samples after 24 hours of each administration,recorded their weights and measured platelet accounts and bleeding tendencies. After two weeks of injection,the mice were sacrificed and dissected,smeared with the bone marrow that had been taken out,counted the number of megakaryocyte under the microscope. Assessed the therapeutic effect of 1,25-dihydroxyvitamin D3 on the model mice in several aspects. Results From the 10 th day of the injection,the food-intake of the treatment group was markedly increased,the spirit status and reaction was gradually recovered; the body weight and platelet account,compared with the model group,were both significantly increased（ P〈0. 05）; bleeding tendency evident was markedly decreased（ P〈0. 05）; the number of the platelet-forming megakaryocyte was higher than the model group and lower than the normal control group,while the number of the megakaryocyte was lower than the model group and higher than the normal control group,the difference was statistically significant. Conclusion 1,25-dihydroxyvitamin D3 shows some influence on the improvement of general status,the rise of body weight and platelet account,the reduction of bleeding tendency and the promotion of platelet＇s production of the model mice.