N-乙酰半胱氨酸对香烟暴露小鼠肺组织中IL-17A及IL-22的影响

Changes of interleukin-17A and interleukin-22 in the lung of cigarette smoke exposed mice and the effect of N-acetylcysteine

目的探讨香烟暴露诱导的肺气肿小鼠肺组织中白介素-17A(IL-17A)以及白介素-22(IL-22)的变化以及戒烟、N-乙酰半胱氨酸(NAC)对IL-17A和IL-22的影响。方法采用香烟暴露法建立小鼠肺气肿模型,给予戒烟及NAC灌胃干预。采用ELISA法检测肺匀浆及肺泡灌洗液(BALF)中IL-17A和IL-22浓度。HE染色观察肺组织病理学变化。结果香烟暴露可使小鼠肺组织发生肺气肿样变化。与正常对照组相比,肺匀浆及BALF中,香烟暴露后IL-17A及IL-22水平升高(P<0.05)。与香烟暴露组比较,戒烟及NAC灌胃后IL-17A及IL-22水平下降(P<0.05)。肺匀浆及BALF中IL-22与IL-17A的比值逐渐降低。结论 IL-17A及IL-22与香烟暴露导致的小鼠肺组织中慢性炎症的发生有关。戒烟及NAC处理对吸烟导致的IL-17A及IL-22变化有一定干预作用。

Objective To investigate the changes of interleukin-17A（ IL-17A） and interleukin-22（ IL-22） in the lung of cigarette smoke exposed mice and the effect of smoking cessation and N-acetylcysteine（ NAC） treatment.Methods BALB / c mice in experimental groups were exposed to cigarette smoke. Then the smoke-exposure was stopped and mice were treated with NAC gavage. The mice were executed 1,2,and 3 months after smoking cessation. Lung tissue sample and bronchoalveolar lavage fluid（ BALF） were collected. HE staining was used to observe the pathologic changes of the lung and ELISA was used to measure the level of IL-17 A and IL-22. Results Considerable emphysematous changes was found in the lung of mice exposed to cigarette smoke. Compared with the controls,the level of IL-17 A and IL-22 elevated remarkably in pulmonary tissue and BALF after smoking exposure and declined gradually after smoking cessation. Additional NAC gavage treatment enhanced the decline tendency.Conclusion IL-17 A and IL-22 might play a complex role in the chronic inflammatory changes of lung in mice exposed to cigarette smoke.