脐血移植患者T淋巴细胞内细胞因子的特性研究

The characteristics of T lymphocyte cytokine in the cord blood transplantation patients

目的探讨异基因脐血移植后外周血T淋巴细胞免疫功能的特点及规律。方法 11例异基因脐血移植患者分别在移植前、移植后30、60和90 d取外周血,将其中T淋巴细胞在莫能霉素存在的情况下,体外经十四烷酰拂波醇乙脂和离子霉素刺激后,分别进行CD4-FITC、CD8-FITC荧光单抗染色和γ-IFN-PE、IL-4-PE荧光单抗胞内染色,最后进行流式细胞仪分析。结果脐血移植后60 d的T淋巴细胞分泌γ-IFN和IL-4的比率开始增加,90 d后T淋巴细胞分泌γ-IFN和IL-4的比率增加明显(P<0.001)。结论脐血移植60 d后患者的免疫功能开始恢复,90 d后免疫功能达到顶峰,脐血移植是治疗急性白血病的一种有效方法。

Objective To evaluate characteristics of T-lymphocyte immunologic function and reconstitution after allo-gene cord blood transplantation. Methods To peripheral blood from 11 human cord blood at 30,60 and 90 days after cord blood transplantation,the T-lymphocytes with phorbol 12-myristate 13-acetate and ionomycin in the presence of monensin. CD4＋and CD8＋Tcells were analyzed for γ-IFN and IL-4 intrecellular production by flow cytometry. Results The rate of γ-IFN and IL-4 from T lymphocytes began to increase at 60 days after the transplantation,and the rate of γ-IFN and IL-4 increased significantly at 90 days after the transplantation（ P〈0. 001）. Conclusion The immune function begins to recover at 60 days after the Cord blood transplantation,the immune function peaks after 90 days. Cord blood transplantation is effective for the acute leukemia trerapy.