sIL-33联合CEA、CA19-9检测对结直肠癌的诊断价值

Diagnostic value of the detection of sIL-33 combined with CEA and CA19-9 in colorectal cancer

目的研究结直肠癌(CRC)患者血清白介素-33(s IL-33)水平,评价其对CRC诊断的临床意义。方法收集121例CRC患者的术前血清标本与临床病理资料,以81例体检健康者、33例肠息肉患者作为对照。用ELISA法检测s IL-33含量,分析其与临床病理参数的关系,并采用ROC曲线、Logistic回归分析比较s IL-33、CEA和CA19-9单独检测或3者联合检测的诊断价值。结果 CRC患者s IL-33含量[172.44(108.08,321.57)pg/m L]显著高于肠息肉组[67.31(37.72,130.44)pg/m L]和体检健康组[71.73(36.80,85.53)pg/m L],差异有统计学意义(P均<0.01)。s IL-33与CRC的分化程度、肿瘤部位有关,差异有统计学意义(P均<0.01)。s IL-33、癌胚抗原(CEA)和糖链抗原19-9(CA19-9)3者联合检测的敏感性(80.99%)、准确率(86.38%)和曲线下面积AUCROC(0.933 4)均比单独检测高,其特异性(92.11%)也处于较高水平,具有较好的诊断价值。结论s IL-33、CEA和CA19-9 3者联合检测对CRC诊断具有较好的临床应用价值,可作为潜在的血清学标志物。

Objective To investigate the level of serum interleukin-33（ s IL-33） in colorectal carcinoma（ CRC） patients and its clinical significance. Methods The preoperative serum samples and clinical pathological data from 121 CRC patients were collected,and81 healthy persons and 33 patients with intestinal polyp were selected as the control. The s IL-33 level was detected using an ELISA method,and its correlation with clinical pathological features was analyzed. The diagnostic values of the detection of s IL-33,CEA and CA19-9 alone or their combination in CRC were investigated by ROC curve and Logistic regression analysis. Results The s IL-33 levels [median（ P25,P75） ]in CRC patients [172. 44（ 108. 08,321. 57） pg / m L]were significantly higher than those in intestinal polyp patients [67. 31（ 37. 72,130. 44） pg / m L]and healthy controls [71. 73（ 36. 80,85. 53） pg / m L]（ P〈 0. 01）. The s IL-33 levels in CRC patients were significantly correlated with the differentiated grade and location of the tumor（ P 〈0. 01）. The sensitivity（ 80.99%）,accuracy（ 86. 38%） and AUCROC（ 0. 933 4） of the combined detection of s IL-33,CEA and CA19-9 were higher than that of their single detection,and its specificity（ 92. 11%） was also in a high level,so it had preferable diagnostic value in CRC. Conclusion The combined detection of s IL-33,CEA and CA19-9,as a new potential serum marker,has preferable value in the diagnosis of CRC.