不同西兰花品种中硫代葡萄糖苷的组分与含量分析

Glucosinolate Component and Content Analysis of Different Broccoli Varieties

采用HPLC法测定分析了29个西兰花品种的硫代葡萄糖苷组分与含量，结果检测到8种硫代葡萄糖苷，包括4种脂肪族硫苷：3-甲基硫氧烯丙基硫苷、2-羟基-3-丁烯基硫苷、4-甲基硫氧丁基硫苷和3-丁烯基硫苷：4种吲哚族硫苷：4-羟基吲哚基-3-甲基硫苷、3-甲基吲哚基硫苷、4-甲氧基吲哚基-3-甲基硫苷和1-甲氧基吲哚基-3-甲基硫苷。总硫苷含量平均为11．27μmol/g，RAA,GBC和NEO是西兰花三种主要的硫苷成分，分别占总硫苷含量的34．78％、25．02％和23．25％。不同西兰花品种的总硫苷含量变异范围5．60-17．04μLm01／g，存在较大差异。

Glucosinolates component and content of 29 broccoli varieties were analyzed by HPLC method. As a result, a total of eight kinds of glucosinolates in broccoli bud-head were detected. Four kinds of them were aliphatic glucosino- lates, such as glucoiberin（IBE）, progoitrin（PRO）, glucoraphanin（RAA） and gluconapin（NAP）, and the others were indolic glucosinolates, such as 4-Hydroxyglucobrassicin（4OH）, glucobrassicin（GBC）, 4-Methoxyglucobrassicin（4ME） and Neoglu- cobrassicin （NEO）. The content of total glucosinolates was 11.27μmol· g^-1 DW. RAA, GBC and NEO were the predomi- nant component of glucosinolates in broccoli bud-head, with a proportion of 34.78%, 25.02% and 23.25% respectively. The content of total glucosinolates in different variety of broccoli varied from 5.60 μmol·g^-1 DW to 17.04 μmol·g^-1 DW.