摘要
目的:研究融合基因TMPRSS2:ERG和前列腺癌病理分级的关系。方法:选取前列腺癌的穿刺标本62例为病例组,同时选择10例良性前列腺增生(BPH)患者为对照组,同时纳入9株前列腺癌细胞株作为对照,采用巢式RT-PCR检测融合基因TMPRSS2:ERG,比较融合基因阳性和阴性患者Gleason评分的差异,Logistic回归法分析TMPRSS2:ERG和前列腺癌病理特征的关系。结果:62例前列腺癌患者中有28例检测出TMPRSS2:ERG融合基因,阳性率为45.16%;10例BPH和9株癌细胞株中均未检测出该融合基因。融合基因TMPRSS2:ERG阳性和阴性患者Gleason评分无显著差异(Z=-0.609,P=0.542),但融合基因阳性患者Gleason主评分显著高于阴性患者(Z=-2.600,P=0.009)。单因素Logistic回归分析显示,筛状结构、泡沫状腺体和印戒癌细胞分别与融合基因TMPRSS2:ERG有关联(OR=6.25,P=0.002;OR=6.666,P=0.023;OR=3.24,P=0.035);多因素Logistic回归分析显示,该融合基因和筛状结构有关(OR=3.750,P=0.033)。结论:TMPRSS2:ERG融合基因和前列腺癌中到高级的病理分级有关。
Objective：To study the relationship between TMPRSS2：ERG gene fusion and the pathological grade of prostate cancer（PCa）.Methods：We collected fresh prostatic tissue samples from 62 patients with PCa and another 10 with benign prostatic hyperplasia（BPH）and included 9 cancer cell strains as the control.We examined the TMPRSS2：ERG fusion gene in the PCa samples by nest RT-PCR,compared the Gleason scores between the TMPRSS2：ERG-positive and-negative cases,and analyzed the association of TMPRSS2：ERG fusion with the pathological features of PCa.Results：The TMPRSS2：ERG fusion gene was detected in 28（45.16%）of the PCa cases,but in none of the 10 BPH cases or the 9 cancer cell strains.No statistically significant differences were found in the Gleason scores between the TMPRSS2：ERG-positive and-negative cases（Z =-0.609,P = 0.542）,but the primary Gleason score was markedly higher in the former than in the latter（Z =-2.600,P = 0.009）.Univariate logistic regression analysis showed that TMPRSS2：ERG was associated with the cribriform growth pattern（OR = 6.250,P = 0.002）,foamy gland morphology（OR = 6.666,P = 0.023）,and signet-ring cells（OR = 3.240,P = 0.035）,but multivariate logistic regression analysis manifested that it was associated with the cribriform growth pattern only（OR = 3.750,P = 0.033）.Conclusion：TMPRSS2：ERG gene fusion was associated with higher pathological grades of prostate cancer.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2015年第10期887-891,共5页
National Journal of Andrology
