VEGF165b和VEGF165在胃癌组织中的表达及与预后的关系

Expressions of VEGF165b and VEGF165 in gastric carcinoma and their correlations with prognosis

目的研究VEGF165b和VEGF165在胃癌及癌旁正常组织中的表达及胃癌组织中VEGF165b m RNA/VEGF165 m RNA与预后的关系。方法分别应用实时RT-PCR和免疫组织化学法检测胃癌及癌旁正常组织中VEGF165b和VEGF165的基因及蛋白表达,并对患者进行2年的随访,比较VEGF165b m RNA/VEGF165 m RNA比值及这两种蛋白表达水平在胃癌及癌旁组织中的变化;同时分析VEGF165b与VEGF165蛋白表达的相关性以及胃癌组织中VEGF165b m RNA/VEGF165 m RNA与预后的关系。结果在胃癌组织中VEGF165b m RNA/VEGF165 m RNA低于癌旁正常组织中的水平;在癌旁正常组织中VEGF165b基因及蛋白表达强于VEGF165,在胃癌组织中VEGF165b基因及蛋白的表达则低于VEGF165,而且两者表达呈负相关关系;此外,在两年内死亡的患者中VEGF165b m RNA/VEGF165 m RNA明显低于生存患者中的比值水平。结论与正常组织相比,胃癌组织中存在VEGF165b向VEGF165基因及蛋白水平上的优势转换,这种变化有望成为治疗胃癌的新靶点。

【Objective】 To investigate the expressions of VEGF165 b and VEGF165 m RNA and proteins in gastric carcinoma and the adjacent normal tissue, and to investigate the correlation between the ratio of VEGF165 b m RNA to VEGF165 m RNA in gastric carcinoma and the patients＇ prognosis. 【Methods】 The expressions of VEGF165 b m RNA and VEGF165 m RNA in gastric carcinoma and the adjacent normal tissue were measured by RT-PCR and the expressions of VEGF165 b and VEGF165 proteins were examined by immunohistochemistry. The relationships between the ratio of VEGF165 b m RNA to VEGF165 m RNA and the patients＇ prognosis were determined. 【Results】 The ratio of VEGF165 b m RNA to VEGF165 m RNA in the gastric carcinoma was lower than that in the adjacent normal tissue. The expressions of VEGF165 b m RNA and protein were higher than those of VEGF165 in the normal tissue. However, the expressions of VEGF165 b m RNA and protein were lower than those of VEGF165 in the gastric carcinoma tissue. There was a negative correlation between the protein expressions of VEGF165 b and VEGF165. Furthermore, the ratio of VEGF165 b m RNA to VEGF165 m RNA in the patients who were dead in two years was lower than that of the survivals.【Conclusions】 Compared with the normal tissue, there is a predominant conversion from VEGF165 b to VEGF165 in both m RNA and protein levels in gastric carcinoma, which can be a new target for treating gastric carcinoma.