右美托咪定与异丙酚对大鼠肠缺血再灌注损伤影响的比较

Comparison of effects of dexmedetomidine and propofol on intestinal ischemia-reperfusion injury in rats

目的比较右美托咪定与异丙酚对大鼠肠缺血再灌注损伤的影响。方法SPF级健康成年雄性Wistar大鼠40只，2～3月龄，体重185～230g，采用随机数字表法，将其分为4组（n=10）：假手术组（S组）、肠缺血再灌注组（I／R组）、异丙酚组（P组）和右美托咪定组（D组）。采用夹闭大鼠肠系膜上动脉20min后恢复灌注的方法建立肠缺血再灌注损伤模型。P组和D组于缺血前20min，分别经股静脉注射异丙酚10mg／kg、右美托咪定5μg／kg。于再灌注2h时采集下腔静脉血样，测定血清二胺氧化酶（DAO）活性，光镜下观察肠黏膜上皮组织病理学结果，进行Chiu评分。结果与s组比较，I／R组、P组和D组血清DAO活性和肠黏膜Chiu评分升高（P〈0．05）。与I／R组比较，P组和D组血清DAO活性和肠黏膜Chiu评分降低（P〈0．05）。与P组比较，D组血清DAO活性和肠黏膜Chiu评分降低（P〈0．05）。结论麻醉剂量右美托咪定减轻大鼠肠缺血再灌注损伤的效应优于异丙酚。

Objective To compare the effects of dexmedetomidine and propofol on intestinal ische- mia-reperfusion （I/R） injury in rats. Methods Forty male SPF Wistar rats, aged 2-3 months, weighing 185-230 g, were randomized into 4 groups （n = 10 each） using a random number table： sham operation group （S group）, intestinal I/R group （I/R group）, propofol group （P group） and dexmedetomidlne group （ D group）. Intestinal I/R was produced by occlusion of the superior mesenterie artery for 20 min fol- lowed by reperfusion. In P and D groups, propofol 10 mg/kg and dexmedetomidine 5 μg/kg were injected, respectively, via the femoral vein at 20 min before occlusion. At the end of 2 h reperfusion, the blood sam- ples were collected from the inferior vena cava for determination of serum diamine oxidase （DAO） activity. A segment of the intestine of 5 cm in length was removed for microscopic examination with light microscope. The degree of damage to intestinal mucous membrane was scored according to Chiu. Results Compared to S group, the activity of DAO and Chiu＇ s score were significantly increased in I/R, P and D groups. Compared to I/R group, the activity of DAO and Chiu＇ s score were significantly decreased in P and D groups. Compared to P group, the activity of DAO and Chiu＇ s score were significantly decreased in group D. Conclusion Anesthetic dose of dexmedetomidine pretreatment reduces intestinal I/R injury in rats, and the effect is superior to that produced by propofol.