四川省献浆员血浆蛋白浓度及白球比分布调查

Investigation of plasma protein concentration and distributions of ratio of albumin to immunoglobulins in plasma donors in Sichuan Province, China

目的调查四川省献浆员血浆蛋白浓度及白球比分布,探讨延长献浆年龄的可行性。方法将2 467份四川省献浆员的血浆样本分别按地域（东北部、中部、南部）、年龄（18~35周岁、36~45周岁、46~55周岁）、性别（男性、女性）、献浆次数（1~26次、27~52次、53~78次、79~110次）、年献浆频次（1~5次、6~10次、11~15次、16~23次）分组,采用《中国药典》三部（2010版）中规定的定量双缩脲法检测不同组别献浆员的血浆蛋白浓度,琼脂糖凝胶电泳分析不同组别献浆员白蛋白与球蛋白比例（白球比）。结果四川省单采血浆公司不同年龄、不同献浆次数、不同年献浆频次献浆员血浆蛋白浓度差异无统计学意义（P均〉0.05）;不同献浆次数、不同年献浆频次献浆员血浆白球比差异无统计学意义（P均〉0.05）;不同年龄的献浆员白球比差异有统计学意义（P〈0.01）,但所有献浆员血浆蛋白质含量均在50 g/L以上,白蛋白含量均占血浆蛋白质含量的50%以上,蛋白质分布中的白球比均在正常的范围内,献浆员每年血浆电泳及每次献血浆的血浆蛋白质含量检测结果均符合国家法规要求。结论结合新版《献血者健康检查要求》及本研究结果,建议国家将献血浆年龄由55周岁延长至60周岁。

Objective To investigate the plasma protein concentration and distributions of ratio of albumin to immunoglobulins in plasma donors in Sichuan Province, China as well as the feasibility of extended age of plasma donors.Methods A total of 2 467 plasma samples from donors in Sichuan Province were grouped according to district（northeast,central and south parts of the province）, age（18 ~ 35, 36 ~ 45 and 46 ~ 55 years）, gender（male and female）, time of donation（1 ~ 26, 27 ~ 52, 53 ~ 78 and 79 ~ 110 times）and annual frequency of donation（1 ~ 5, 6 ~ 10, 11 ~ 15 and16 ~ 23 times） separately, in which the plasma protein concentration was determined by quantitative biuret method specified in Chinese Pharmacopoeia（Volume Ⅲ, 2010 edition）, and the ratio of albumin to immunoglobulins by agarose gel electrophoresis. Results The plasma protein concentrations based on age as well as time and annual frequency of donation showed no significant difference（each P 〉 0. 05）. The ratio of albumin to immunoglobulins based on time and annual frequency of donation showed no significant difference（each P 〉 0. 05）, while that based on age showed significant difference（P 〈 0. 01）. However, all the plasma protein of the donors reached concentrations of more than 50 g / L, of which albumin accounted for more than 50%. All the ratios of albumin to immunoglobulins in protein were within the normal range. The test results of plasma by electrophoresis each year and protein content at each donation met the relevant national requirements. Conclusion Based on the Requirements for Physical Examination of Blood Donor and the result of this study, it is recommended that the upper limit of age of plasma donors may be extended from 55 to 60 years.