乙型肝炎患者血清B7-H3和IFN-γ的水平及临床意义

Serum levels of B7-H3 and IFN-γ in patients with hepatitis B and clinical implications

目的检测乙型肝炎患者血清可溶性B7-H3(s B7-H3)和γ干扰素(IFN-γ)的水平,并探讨其临床意义。方法选取87例乙型肝炎患者,包括急性肝炎16例、慢性中至重度肝炎25例、肝硬化代偿期24例和肝硬化失代偿期22例,同期24例体检健康者为正常对照。采用ELISA检测乙型肝炎患者和对照人群血清s B7-H3和IFN-γ的含量,分析二者分别与天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、甲胎蛋白(AFP)、白细胞(WBC)计数及HBV DNA定量结果的相关性。结果正常对照组血清s B7-H3、IFN-γ水平为(27.82±12.75)ng/m L、(328.84±240.20)pg/m L,乙型肝炎患者血清s B7-H3、IFN-γ含量分别为(202.17±58.14)ng/m L和(3436.11±1605.01)pg/m L,均显著高于正常对照组。s B7-H3与IFN-γ密切相关,AST、ALT、AFP、WBC及HBV DNA与二者均无相关性。s B7-H3在急性肝炎时水平最高,IFN-γ则在慢性肝炎时水平最高。结论不同临床类型乙型肝炎患者的s B7-H3、IFN-γ含量明显高于正常者,连续监测血清s B7-H3、IFN-γ可能对乙型肝炎患者的疾病转归预测有重要临床价值。

Objective To detect the serum levels of soluble BT-H3 （sB7-H3） and interferon γ （IFN-γ） in patients with hepatitis B and related clinical implications. Methods The levels of sB7-H3 and IFN-γ were detected by ELISA in 87 cases of hepatitis B including 16 cases of acute hepatitis, 2.5 cases of chronic moderate to severe hepatitis, 2.4 cases of liver cirrhosis, 22 cases of decompensated cirrhosis, and 24 healthy subjects. Their correlations with the levels of aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, α-fetoprotein （AFP）, white blood cells （WBCs）, hepatitis virus B （HBV） DNA were analyzed statistically. Results The serum levels of sB7-H3 and IFN-γ in patients with hepatitis B were （202.17 ± 58.14） ng/mL and （3436.11 ±1605.01） pg/mL, respectively, which were significantly higher than those in the healthy subjects. SBT-H3 was closely related to IFN-γ. AST, ALT, AFP, WBCs, and HBV DNA had no correlations with sBT-H3 and IFN-γ. SB7-H3 was the highest in the patients with acute hepatitis, while IFN-γ, was the highest in the patients with chronic hepatitis B. Conclusion The levels of sB7-H3 and IFN-γ in the patients with hepatitis B are higher than those in the healthy subjects. Continuous monitoring of serum sB7-H3 and IFN-γ may helpful in predicting disease outcomes for patients with hepatitis B.