CXC趋化因子受体3的单克隆抗体抑制体外培养的MCF-7细胞和HepG2细胞的增殖及迁移

CXCR3 monoclonal antibody inhibits the proliferation and migration of MCF-7 cells and HepG2 cells in vitro

目的研究CXC趋化因子受体3(CXCR3)单克隆抗体(mAb)对MCF-7人乳腺癌细胞和HepG2人肝癌细胞体外增殖及迁移的影响。方法诱生腹水法制备CXCR3 m Ab;流式细胞术筛选高表达CXCR3的MCF-7细胞和HepG2细胞;MTT法检测CXCR3 mAb对MCF-7细胞和HepG2细胞增殖及干扰素诱导的T细胞α趋化因子(I-TAC)对MCF-7细胞和HepG2细胞促增殖的影响;TranswellTM法研究CXCR3 mAb对MCF-7细胞和HepG2细胞迁移及I-TAC对MCF-7细胞和HepG2细胞促迁移的影响。结果 MCF-7细胞和HepG2细胞CXCR3的阳性表达率分别为83.5%和96.2%;50μg/m L CXCR3 m Ab能够显著的抑制MCF-7细胞和HepG2细胞的增殖并抑制I-TAC对MCF-7细胞和HepG2细胞的促增殖作用;50μg/m L CXCR3 m Ab可抑制MCF-7细胞和HepG2细胞的迁移,并抑制I-TAC对MCF-7细胞和HepG2细胞促迁移作用。结论 CXCR3 mAb能够显著地抑制高表达CXCR3的肿瘤细胞的增殖及迁移。

Objective To study the effects of chemokine （C-X-C motif） receptor 3 （CXCR3） monoclonal antibody （mAb） on the proliferation and migration of MCF-7 and HepG2 cells in vitro. Methods Ascites of CXCR3 mAb was prepared at first. MCF-7 and HepG2 cells with high expressions of CXCR3 were screened by flow cytometry. MTT assay was used to detect the effects of CXCR3 mAb on the proliferation of MCF-7 and HepG2 cells in vitro in the absence/presence of interferoninducible T-cell alpha chemoattractant （I-TAC）. Transwell^TM assay was performed to investigate the effects of CXCR3 mAb on the migration of MCF-7 and HepG2 cells in vitro in the absence/presence of I-TAC. Results The expression rate of CXCR3 on MCF-7 and HepG2 cells were 83.5% and 96.2%, respectively. 50 μg/mL CXCR3 mAb significantly inhibited the proliferation and migration of MCF-7 and HepG2 cells, and also inhibited the promoting effect of I-TAC on the proliferation and migration of MCF-7 and HepG2 cells in vitro. Conclusion CXCR3 mAb can significantly inhibit the proliferation and migration of the tumor ceils highly expressing CXCR3 in vitro.