柚(Citrus grandis Osbeck cv.)MADS box基因的表达分析

The Analysis of MADS box Genes Expressing in the Citrus grandis Osbeck cv.

以De novo转录组学方法分析柚(Citrus grandis Osbeck cv.)MADS box基因家族的基因.以特早熟龙柚(变异型)和琯溪蜜柚(参照型)嫩果(不超过5天)和新叶为材料,经过RNA提取、反转录、测序、组装得到Unigene序列.Unigene序列在Nr、Swiss-Prot、KEGG和COG四大数据库中进行比对,获得MADS box转录因子家族基因注释结果.采用数字基因表达标签(digital gene expression tag,DGE)技术,对样本中基因转录丰度以RPKM值表示,比较处理组和对照组的RPKM值,以倍性变化≥2确定差异表达基因(differentially expressed genes,DEGs).从特早熟龙柚和琯溪蜜柚嫩果和叶片中,总共注释到28个MADS box基因家族基因.在龙柚嫩果(FE)和蜜柚嫩果(FL)中表达的有25个,其中有6个属于差异表达基因.在龙柚叶片(LE)和蜜柚叶片(LL)中表达的有27个,其中有7个属于差异表达基因.注释到的28个MADS box基因家族基因在克莱门特柚(Citrus clementina)和中国甜橙(Citrus sinensis)报道过的分别有18个和20个,其中有5个基因既在中国甜橙和克莱门特柚都没有报道过,也没有发现在其它Citrus物种报道过.

This paper is concerning the MADS box gene family from the fruitlet（less than 5 days） and the yang leaf of two kinds of pomelo breeding（Citrus grandis Osbeck cv.） using the De novo transcriptomics method. The fruitlet and the yang leaf from the Early Mature Dragon Pomelo（which was taken as mutant group）and the Guangxi Sweet Pomelo（which was taken as reference group）were taken as experiment materials, by RNA extraction, reverse transcription, sequencing and assembly Unigene sequences. The MADS box transcription factor family gene annotation results were retrieved from the Nr,Swiss Prot, KEGG and COG the four databases. Adopting digital gene expression label（DGE） technology, the gene transcription abundance in the sample was taken as RPKM value, that the differentially expressed genes（DEGs） were identified by comparing the different of the RPKM value between the mutant group and reference group, with the duplication≥2. There were 28 MADS box genes were annotated from the fruitlet and the yang leaf of the Early Mature Dragon Pomelo and the Guangxi Sweet Pomelo. There were 25 MADS box genes expressed in the fruitlet from the Early Mature Dragon Pomelo（FE） and the Guangxi Sweet Pomelo（FL）, and sixs of them were DEGs. Meanwhile, There were 27 MADS box genes expressed in the leaf from the Early Mature Dragon Pomelo（LE） and the Guangxi Sweet Pomelo（LL）, and sevens of them were DEGs. The 28 MADS box gene family was reported eighteens of them in Citrus clementina and twenties of them in Citrus sinensis, respectively, and fives of them have reported neither in Citrus clementina nor in Citrus sinensis, and have not found in the Citrus＇ s other breeding.