双层纳米金修饰电极对多巴胺的测定及应用研究

Determination of dopamine at double layer of gold nanoparticles modified glassy carbon electrode and its application

利用含有连续腺嘌呤碱基模块的DNA（CA DNA）构建了双层纳米金,在此基础上制备了由聚番红花红和双层三维分布的纳米金修饰的玻碳电极,并研究了多巴胺（DA）在此修饰电极上的电化学行为,发现对于DA的氧化,CA DNA构建的电极比巯基DNA构建的电极能够起到更明显的电催化作用。利用差分脉冲法（DPV）考察了DA测定的优化条件,并发现其浓度在8.0×10^-9~1.2×10^-6mol·L-1范围内与氧化峰电流呈良好的线性关系。该电极用于实际样品的测定,结果满意。

DNA with consecutive adenine block（ CA DNA） was firstly applied to construct three-dimensional distributed gold nanaoparticles（ 3D GNPs）. Based on this,3D GNPs,which were anchored with poly（ safranine T） and DNA,modified electrode was prepared. The electrochemical behavior of dopamine（ DA） at this modified electrode was also investigated. It was found that the CA DNA modified electrode shows a better electrocatalytic activity for the oxidation of DA than that at mercapto-DNA. The optimum conditions of DA determination were studied by differential pulse voltammetry（ DPV） and it was found that the linear relationship between oxidation peak current and concentration was in the linear range of 8. 0× 10^-9~ 1. 2× 10^-6mol·L-1. The modified electrode can be applied to the determination of DA in the practical injection samples with satisfactory results.