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应用PCR法快速检测公共场所中分枝杆菌 被引量:3

Rapid detection of Mycobacterium tuberculosis in the public places by PCR method
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摘要 目的调查深圳市公共场所中分枝杆菌的污染状况。方法于2012年11月采集商场、宾馆和综合性医院中水、土壤、风管积尘和气溶胶样本,巢式PCR方法扩增结核分枝杆菌复合群IS6110和分枝杆菌Hsp65基因,琼脂糖凝胶电泳检测扩增产物。结果深圳公共场所中水、土壤、风管积尘和气溶胶样本中结核分枝杆菌复合群阳性率分别为4.68%、0.97%、5.41%和3.85%;分枝杆菌阳性率分别为21.1%、4.85%、17.1%和13.1%。结论结核杆菌复合群在宾馆及商场空调积尘、自来水和空调冷却水中阳性率较高,分枝杆菌在空调冷却水、自来水和综合性医院气溶胶及空调积尘中阳性率较高。应引起重视,强化防控措施,保护人群健康。 Objective To investigate the contamination status of Mycobacterium tuberculosis in the public places of Shenzhen City. Methods The samples of water,soil,duct dust and aerosol were collected from malls,hotels and general hospitals in November 2012. Nested- PCR based on IS6110 and Hsp65 gene was used to detect Mycobacterium tuberculosis complex and mycobacteria respectively. The amplification products were detected by agarose gel electrophoresis. Results The positive rates of Mycobacterium tuberculosis complex in the samples of water,soil,duct dust and aerosol were 4. 68%,0. 97%,5. 41% and 3.85% respectively. The positive rates of mycobacteria were 21. 1%,4. 85%,17. 1% and 13. 1% respectively. Conclusions Mycobacterium tuberculosis complex is widely distributed in duct dust of air- conditioners,tap water and cooling water of air-conditioners in malls and hotels. The positive rates of mycobacteria in air- conditioners' cooling water,tap water and general hospitals' aerosol and duct dust are higher. Thus it is necessary to pay more attention to intensifying prevention and control measures so as to protect the people's health.
出处 《实用预防医学》 CAS 2015年第11期1304-1307,共4页 Practical Preventive Medicine
基金 卫生行业专项任务(201002001)
关键词 分枝杆菌 公共场所 聚合酶链反应 Mycobacterium tuberculosis Public place Polymerase chain reaction
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