不同产地及干燥方式板蓝根中4种活性成分含量比较分析

Comparison of Four Active Compounds in Radix Isatidis of Different Origins and Drying Methods

目的：建立板蓝根中表告依春、腺苷、靛蓝和靛玉红含量测定的方法。方法：采用Agilent 5 TC-C18色谱柱（250 mm×4.6 mm,5μm）,甲醇-水为流动相梯度洗脱,流速为1 m L·min-1;双波长切换时间序列采样：0~20 min为254 nm,20~40 min为285 nm;柱温30℃。结果：在上述色谱条件下,测得表告依春,腺苷,靛蓝,靛玉红的线性范围分别为0.100~0.600μg（r=0.9998）,0.122~0.732μg（r=0.9998）,0.024~0.144μg（r=0.9998）,0.004~0.024μg（r=0.9998）与峰面积呈良好的线性关系。结论：各产地中四种成分均有显著性差异,表告依春和腺苷的含量相对靛玉红、靛蓝较高;不同干燥方式对四种成分的影响较大,尤其不适合高温干燥。该含量测定的方法简单、准确、重复性良好,可为板蓝根药材的质量控制提供一定的依据。

Objective： To establish a method for determining epigoitrin,adenosine,indigo and indirubin in Radix isatidis. Methods： Four components were separated by Agilent C18column（ 4. 6 mm × 250 mm,5μm） with gradient mobile phase of methanol-water at the flow rate of 1 m L·min- 1. The detection wavelength was set at 254 nm（ 0- 25 min） and 285 nm（ 30- 40 min）.The column temperature was maintained at 30 ℃. Results： The calibration curves of epigoitrin,adenosine,indigo and indirubin were in good linearity over the range of 0. 100- 0. 600 μg（ r = 0. 9998）,0. 122- 0. 732 μg（ r = 0. 9998）,0. 024- 0. 144 μg（ r =0. 9998） and 0. 004- 0. 024 μg（ r = 0. 9998） and the peak area showed a good linear relationship. Conclution： There are significant differences among all the four components in Radix Isatidis from different habitats and the content of epigoitrin and adenosine is higher than that of indigo and indirubin. The influence of different drying methods on the four components is very large,especially not suitable for high temperature drying. The determination method is simple,accurate and reproducible for quality control of Radix Isatidis. Conclusion：