藤黄健骨片对骨质疏松模型大鼠干预作用实验研究

Experimental Study on Intervention of Tenghuang Jiangu Tablets on Osteoporosis Model Rats

目的:观察藤黄健骨片对地塞米松和维甲酸所致骨质疏松模型大鼠的改善作用。方法:采用地塞米松肌肉注射和维甲酸灌胃的方法制成骨质疏松模型大鼠,随机分为5组(模型组、依普黄酮片组、藤黄健骨片低、中、高剂量组),另单设空白对照组。连续给药14天,观察血液生化指标(钙、碱性磷酸酶)、血清激素水平(雌二醇、降钙素)和骨力学的变化情况。结果:藤黄健骨片高剂量组能明显提高大鼠血清钙和降钙素水平,且降低其血清碱性磷酸酶水平;中、高剂量组能明显提高血清雌二醇水平;藤黄健骨片低、中、高剂量组能明显提升雄性大鼠的最大弯曲力,高剂量组能明显提升雌性大鼠的最大弯曲力;与模型组比较藤黄健骨片低、中、高剂量组的抗弯强度和断裂挠度均有显著升高(P<0.05)。结论:藤黄健骨片对地塞米松和维甲酸诱导的骨质疏松动物模型具有显著的改善作用,能提高血清钙和激素水平,并能明显改善骨力学特性。

Objective ： To observe the improvement of Tenghuang Jiangu Tablet on the osteoporosis model rat induced by dexamethasone and tretinoin. Method： Sprague-Dawley rats were injected intramuscularly with dexamethasone and lavaged with tretinoin to be osteoporosis models. The model rats were divided randomly into five groups （ model group, ipriflavone tablet group, groups with low, middle and high dosage of Tenghuang Jiangu Tablet ） . The blank control group was set separately. The dosing course was 14 days. The serum biochemical indexes, the level of serum hormone and the changes of bone mechanics were detected and observed. Result： The high dosage group of Tenghuang Jiangu Tablet could improve the level of serum calcium and calcitonin significantly and could reduce the level of serum alkaline phosphatase. The middle and high dosage groups of Tenghuang Jiangu Tablet could improve the level of serum estradiol. The low, middle and high dosage groups of Tenghuang Jiangu Tablet could significantly improve maximum bending force of male rats and the high dosage group of Tenghuang Jiangu Tablet could significantly improve maximum bending force of female rats. Compared with that of the model group,the bending strength and fracture deflection of the low, middle and high dosage groups increased significantly （P〈0.05） .Conclusion： Tenghuang Jiangu Tablet has the significant effect on rat osteoporosis model, which could improve serum calcium, serum hormone and bone mechanical properties.