艾烟与香烟对载脂蛋白E基因敲除小鼠自主行为与海马GFAP表达的影响

Effects of Moxa Smoke Versus Tobacco Smoke on Autonomous Behaviors and Hippocampal GFAP Expression in Apolipoprotein E Gene Knockout Mice

目的观察艾烟与香烟对载脂蛋白E基因敲除（Apo E-/-）小鼠自主行为能力与海马中胶质纤维酸性蛋白（GFAP）表达的影响。方法将13只8周龄C57BL/6小鼠作为空白对照组,27只同龄Apo E-/-小鼠随机分为Apo E-/-模型组、艾烟组、香烟组。香烟组与艾烟组中小鼠暴露于5~15 mg/m3烟气环境。各组小鼠每天干预20 min,每星期6 d,共干预12星期。于第13星期进行行为学测试,之后处死动物、取材,对脑组织海马中GFAP进行免疫组化法检测。结果空白组小鼠自主活动明显高于模型组小鼠自主活动（P〈0.05）;香烟组没有表现出与模型组、艾烟组的明显差异（P〉0.05）,但低于空白组（P〈0.05）。艾烟组移动距离高于模型组（P〈0.05）,站立次数低于空白组。模型组小鼠海马中GFAP免疫反应产物积分光密度明显高于空白组与艾烟组（P〈0.05）。香烟组小鼠海马GFAP表达高于艾烟组与空白组（P〈0.05）。结论艾烟可能增强小鼠中枢神经系统的兴奋性,减低阿茨海默病模型小鼠海马中GFAP的表达。

Objective To investigate the effects of moxa smoke versus tobacco smoke on autonomous behaviors and hippocampal expression of glial fibrillary acidic protein（GFAP） in apolipoprotein E gene knockout（Apo E-/-） mice. Method Thirteen 8-week-old C57BL/6 mice constituted a blank control group. Twenty-seven Apo E-/- mice of the same age were randomized into Apo E-/- model, moxa smoke and tobacco smoke groups. The tobacco smoke and moxa smoke groups of mice were exposed to smoke 5-15 mg/m3 circumstances. Every group of mice was intervened in 20 min. daily, six days a week, for a total of 12 weeks. A behavioral test was conducted in week 13. The animals were then sacrificed to take the materials. Hippocampal GFAP in the brain was measured by an immunohistochemical method. Result Autonomous activities were significantly more in the blank group than in the model group（P0.05） and significantly fewer in the tobacco smoke group than in the blank group（P0.05）. There was no statistically significant difference in autonomous activities between the tobacco smoke group and the model or moxa smoke group（P0.05）. Moving distance was longer in the moxa smoke group than in the model group（P0.05）. Standing-up number was smaller in the moxa smoke group than in the blank group（P0.05）. Integral optical density of GFAP immune reaction products in the hippocampus was significantly higher in the model group of mice than in the blank and moxa smoke groups（P0.05）. Hippocampal GFAP expression was significantly higher in the group of mice than in the moxa smoke and blank groups（P0.05）. Conclusion Moxa smoke can increase the excitability of central nervous system in mice and reduce hippocampal GFAP expression in a mice model of Alzheimer disease.