高效液相色谱法同时测定化妆品中氢醌、苯酚和防腐剂等10种限用物质

Determination of 10 Kinds of Restricted Substances of hydroquinone, phenol and preservatives in Cosmetics by HPLC

建立了高效液相色谱法同时检测化妆品中氢醌、苯酚和防腐剂等10种物质的方法,以甲醇-乙腈-0.025 mol/L磷酸二氢钠（pH=3.80）为流动相,梯度洗脱,10种物质在0.2~200μg/m L范围内线性良好（R2〉0.9990）,平均回收率在84.15%~101.02%之间,RSD在0.91%~3.84%,精密度良好。此方法灵敏度高、准确性好、实用性强,适合检测化妆品中氢醌、苯酚和防腐剂等。

A high performance liquid chromatography（HPLC） coupled with photodiode array detector（DAD） method was developed for the determination of content of hydroquinone, phenol and preservatives in cosmetics sample. By using the methanol-acetonitrile-0.025 mol/L Na H2PO4（pH=3.80） as mobile phase, gradient elution to determine 10 kinds of restricted substances in cosmetics. They showed good linearity with the range of 0.2~200 μg/m L（R2〉0.9990）, average recovery was 84.15 %~101.02 % and the relative standard deviations（RSD） ranged from 0.91 % to 3.84 %. The method was sensitive, accurate and rapid and could be applied for the requirements for determination of hydroquinone, phenol and preservatives in Cosmetics.