口腔癌患者VHL表达与上皮间质转化过程的相关性研究

Relationship between VHL expression and epithelial-mesenchymal transition progress in patients with oral cavity cancer

目的探讨口腔癌患者抑癌基因von Hippel Lindau(VHL)的表达与上皮间质转化(epithelial-mesenchymal transition,EMT)相关蛋白表达的相关性。方法选取2010年1月至2014年12月口腔鳞状细胞癌患者80例作为研究对象,应用免疫组织化学方法检测癌组织及癌旁组织VHL基因及EMT相关蛋白Snail蛋白、上皮型钙黏附素(E-cadherin)、神经型钙黏附素(N-cadherin)的表达,分析各因子间相关性及预后危险因素。结果 VHL基因在口腔癌组织中的阳性表达率52.50%,明显低于癌旁组织的阳性表达率85.00%(P<0.05)。VHL表达与E-cadherin表达呈正相关(r=0.848,P<0.01),与Snail蛋白、N-cadherin表达呈负相关(r=-0.313,P<0.01;r=-0.373,P<0.01)。VHL的表达与淋巴结转移、肿瘤分化程度及肿瘤TNM分期相关(P<0.05或P<0.01)。Cox回归模型分析显示淋巴结转移、分化程度及VHL表达是口腔癌预后的独立危险因素(P<0.05或P<0.01)。结论 VHL可能通过调控EMT相关蛋白的表达从而影响口腔癌的发生、发展,VHL低表达可能促进了肿瘤的恶性分化及淋巴转移,且是影响口腔癌预后的独立危险因素。

Objective To investigate the correlation between the expression of yon Hippel Lindau （VHL） , an anti-onco- gene, and epithelial-mesenchymal transition （EMT）in patients with oral cavity cancer. Methods Eighty patients with oral cavity squamous cell carcinoma treated in our hospital from January 2010 to December 2014 were selected as research ob- jects. The expression levels of VHL gene and EMT-related proteins including snail protein, epithelial type cadherin （E-cad- herin） and nerve type cadherin （N-cadherin）in cancer tissues and para-carcinoma tissues were detected by immunohisto- chemisty method. The correlation among various factors and prognostic risk factors were analyzed. Results The positive ex- pression rate of VHL in tumor tissues was significantly lower than that in para-carcinoma tissues （52.50% vs 85.00% ,P 〈 0.05 ）. The VHL expression was positively correlated with the E-cadherin expression （ r = 0. 848 ,P 〈 0.01 ） and negatively correlated with Snail protein and N-cadherin expressions （ r = - 0, 313, P 〈 0. 01 ; r = - 0. 373, P 〈 0.01 ）. The expression of VHL was associated with lymph node metastasis, tumor differentiation degree and tumor TNM classification（ P 〈 0.05 or P 〈0.01 ）. Cox regression model analysis showed that VHL was the independent risk factor influencing prognosis of oral cavity cancer. Conclusion vHL might influence the occurrence and progress of oral cavity cancer by regulating EMT-re- lated proteins. The low-expression of VHL might promote malignant differentiation and lymph node metastasis of oral cavity cancer. VHL is the independent risk factor influencing prognosis of oral cavity cancer.