摘要
目的了解不同分期喉癌患者手术前后的肿瘤-睾丸抗原(CTA)家族中的黑色素瘤抗原-A1(MAGE-A1)和黑色素瘤抗原-A3(MAGE-A3)的血清学浓度变化情况,筛选喉癌诊断的CTA血清标志物。方法采用酶联免疫吸附试验(ELISA)法检测25例喉癌患者手术前、手术后血清中的MAGE-A1和MAGE-A3水平,并进一步检测46例喉癌患者手术前组、33例喉癌患者手术后组和20名健康人血清中的5种CTA。结果 ELISA法检测25例喉癌患者手术前、手术后血清中MAGE-A1和MAGE-A3浓度差异有统计学意义(P<0.05)。在46例喉癌患者手术前组、33例喉癌患者手术后组和20名健康人中,MAGE-A1和MAGE-A3手术前组和健康人组血清的浓度差异有统计学意义(P<0.05)。结论 MAGE-A1和MAGE-A3有可能成为喉癌诊断和预测复发的CTA血清标志物。
Objective To investigate the serological concentration changes of melanoma antigen-A1 （MAGE-A1） and melanoma antigen-A3 （MAGE-A3） of the cancer-testis antigen （CTA） family in patients with laryngeal carci-noma at different stages before and after the operation, and to screen the CTA serum markers of the laryngeal carcino-ma diagnosis. Methods Enzyme-linked immunosorbent assay （ELISA） was used to determine the serum MAGE-A1 and MAGE-A3 in 25 patients with laryngeal cell carcinoma （LCC） before and after the operation. Then, 5 CTA serum markers were determined in the preoperative LCC group （n=46）, postoperative LCC group （n=33） and normal group （n=20）. Results ELISA showed that there were significant differences between preoperative and postoperative serum MAGE-A1 and MAGE-A3 concentration in 25 LCC patients （P〈0.05）. Among the preoperative LCC group （n=46）, postoperative LCC group （n=33） and normal group （n=20）, there were significant differences in serum MAGE-A1 and MAGE-A3 concentration between the preoperative groups and normal group （P〈0.05）. Conclusion MAGE-A1 and MAGE-A3 may become CTA serum markers for the LCC diagnosis and prediction of LSCC recurrence.
出处
《中国药物与临床》
CAS
2015年第10期1381-1384,共4页
Chinese Remedies & Clinics
基金
国家自然科学基金(30772405)
山西医科大学校科技创新基金(01201319)
