乳腺疾病患者血清中IFN-γ的检测及临床意义

The detection and clinical significance of IFN-γ in breast diseases

目的:检测乳腺疾病患者及对照组患者血清中IFN-γ的水平,分析其临床意义。方法:收集2014年3月至2015年3月我院转移性乳腺癌患者30例、局部乳腺癌患者35例、乳腺不典型增生患者29例、乳腺纤维瘤患者31例、正常人群30例。应用酶联免疫吸附试验(ELISA法)定量检测IFN-γ在不同组织血清中的水平。比较乳腺癌组与其他各组间血清IFN-γ水平的差异,同时分析乳腺癌组血清中IFN-γ水平与临床病理特征间的关系,探讨检测IFN-γ的临床意义。结果:转移性乳腺癌患者血清中IFN-γ水平明显高于局部乳腺癌组、乳腺不典型增生组、乳腺纤维瘤组及正常人群组,经统计学分析差异均具有统计学意义(F=142.48,P<0.05)。乳腺不典型增生组、乳腺纤维瘤组及正常人群组血清中IFN-γ的组间表达差异无统计学意义(P>0.05)。乳腺癌患者中IFN-γ的表达水平与年龄和肿瘤大小无关(P>0.05),而与TNM分期、组织学分级、淋巴结状态及脉管浸润有关,差异具有统计学意义(P<0.05)。结论:IFN-γ血清水平与乳腺癌发生及侵袭转移相关,可作为一个潜在的乳腺癌血清标志物。

Objective： To detect the level of IFN - γ, in breast disease patients and control group, analyze its clini- cal significance. Methods ： Collect 30 metastatic breast cancer patients＇ serum, 35 patients＇ serum of localized breast cancer, 29 patients＇ serum of atypical hyperplasia of the breast,31 mammary gland fibroma patients＇ serum and 30 nor- mal people＇s serum. Use the ELISA method to quantitatively detect the level of IFN -γ. Results：The level of IFN -γ in metastatic breast cancer patients＇ serum was apparently higher than localized breast cancer group, atypical hyperpla- sia of the breast group ,mammary gland fibroma group and normal population group （ F = 142.48 ,P 〈0.05 ）. The lev- els of IFN - γ in atypical hyperplasia of the breast group, mammary gland fibroma group and normal population group had no difference （ P 〉 0. 05 ）. There was no relationship among age, tumor size and the expression level of IFN - γ, in breast cancer patients （ P 〉 0. 05 ）, but among TNM staging, histological grade, node status and vascular lymphatic in- vasion, the differences were significant （ P 〈 0.05 ）. Conclusion： IFN - γ is related to the genesis and invasion of breast cancer,it is a potential biomarker for breast cancer diagnosis.