结直肠癌淋巴结转移与泛素结合酶UbcH10的表达

Lymph node metastasis of colorectal cancer and expression of UbcH10

目的检测结直肠癌中泛素结合酶UbcH10的表达,并探讨其与结直肠癌患者临床病理特征和淋巴结转移的相关性。方法采用免疫组织化学法检测结直肠癌组织及正常组织中UbcH10蛋白的表达量,分析其与结直肠癌患者临床病理特征的关系。采用Matrigel侵袭实验分析UbcH10对结直肠癌HT-29细胞株侵袭能力的影响。结果 UbcH10蛋白在正常组织细胞中几乎不表达,在结直肠癌组织的细胞核中高表达,并且表达水平与肿瘤的分化程度、淋巴结转移相关(P<0.05)。Matrigel侵袭实验结果显示UbcH10过表达可显著增加HT-29细胞的体外侵袭能力。结论 UbcH10基因在结直肠癌患者肿瘤组织中过度表达,并与结直肠癌的疾病进展密切相关,可作为结直肠癌诊断和淋巴结转移的潜在肿瘤标志物及结直肠癌基因治疗的新靶点。

Objective To detect the expression of UbcH10 in colorectal cancer（CRC） ,and to investigate its correlation with the clinicopathological characteristics and lymph node metastasis .Methods The expression levels of UbcH10 in CRC tissues and the normal tissues in patients with CRC were detected by immunohistochemistry .Its cor‐relation with the clinicopathological characteristics were analyzed .The influence of UbcH10 overexpression on the in‐vasive ability of HT‐29 cells was detected by using the Matrigel invasion assay .Results The UbcH10 protein was hardly expressed in normal control colorectal tissues ,but highly expressed in the cell nucleus of CRC tissues ,moreo‐ver its expression level was correlated with the differentiation degrees and lymph node metastasis of CRC （P＆lt;0 .05） . The result of the Matrigel invasion assay showed that the UbcH10 overexpression could significantly increase the in vitro invasive ability of HT‐29 cells .Conclusion UbcH10 gene is overexpressed in the tumor tissues of CRC pa‐tients ,and is closely correlated with the progression of CRC ,which may serve as a potential tumor marker of CRC di‐agnosis and lymphnode metastasis ,and a new target point of CRC gene therapy .