基于核酸检测诊断结核分枝杆菌感染的研究进展

Research progress in molecular biological diagnosis of tuberculosis infection

结核病是由结核分枝杆菌感染而引起的传染性疾病。实验室诊断技术的不断进步,为结核病的诊断及治疗提供了多种选择方案。核酸检测是利用分子生物学的理论和技术,通过直接探查核酸的存在状态,从而对人体的状态与疾病做出诊断的方法。近年来,核酸诊断技术的发展极大地推动了实验室的快速诊断,本文就结核杆菌感染后细菌核酸及宿主微小核糖核酸检测方面的新方法、新技术进行综述。

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. The rapid development of laboratory diagnostic technology provides a variety of options for diagnosis and treatment of tuberculosis. Nucleic acid detection is to use the theory and technology of molecular biology, through direct detection of status or defect of particular segments, then make diagnosis of the state of human body and disease. In recent years, the development of nucleic acid diagnostic technique has greatly promoted the rapid diagnostic of laboratories. This article reviews the new methods of bacterial nucleic acid and host mi- croRNAs detection technologies.