超声心动图在构建舒张性心力衰竭模型兔过程中的应用价值

Application value of echocardiography in establishing rabbit models of diastolic heart failure

背景:相关医学研究表明,与收缩性心力衰竭相比,单纯性舒张性心力衰竭的发病率更高。目的:探讨超声心动图在兔单纯性舒张性心力衰竭动物模型监测中的应用价值。方法:选取60只健康新西兰雄性兔,运用腹主动脉缩窄法建立压力负荷性心肌肥厚模型兔,并设假手术组作对照,对各组兔进行超声心动图监测和左心导管检查,对其左室舒张末压和松弛时间常数进行测定,以对单纯性舒张性心力衰竭的存在情况进行有效判断。结果与结论:与假手术组相比,压力负荷性心肌肥厚模型兔建模前、建模后1,4,8周的主动脉瓣环径均明显变短(P<0.05);建模后8周模型兔心房收缩期逆向血流最大流速降低(P<0.05);建模后8周的左室干质量,左室干质量与体质量的比值、左室后壁厚度、收缩压、室间隔、左室收缩压和左室舒张末压水平均明显增高(P<0.05)。结果证实,实验成功建立了单纯性舒张性心力衰竭模型兔,超声心动图可有效监测心肌肥厚时的左室收缩和舒张功能。

BACKGROUND： Related medical researches have shown that the incidence of simple diastolic heart failure is higher than that of systolic heart failure. OBJECTIVE： To investigate the application value of echocardiography in the monitoring of rabbit models of simple diastolic heart failure. METHODS： Sixty healthy male New Zealand rabbits were included in this study. The pressure overload hypertrophy rabbit model was established by the method of abdominal aortic coarctation. Rabbits were randomly divided into surgical group and sham group. All rabbits were subjected to the echocardiography and left heart catheterization, the left ventricular end-diastolic pressure and relaxation time constants were measured to judge the presence of simple diastolic heart failure. RESULTS AND CONCLUSION： Compared with the sham group, the aortic annular diameter was significantly reduced before surgery and at 1, 4, 8 weeks after surgery in the model group（P〈0.05）; the peak of retrograde velocity at atrial contraction was decreased in the model rabbits at 8 weeks after surgery（P〈0.05）; the left ventricle dry weight, ratio of left ventricle dry weight/body weight, left ventricular posterior wall thickness, systolic pressure, interventricular septum, left ventricle systolic pressure, and left ventricular end-diastolic pressure were obviously increased at 8 weeks after surgery（P〈0.05）. Experimental findings indicate that rabbit models of simple diastolic heart failure are successfully established, and echocardiography can effectively monitor the left ventricular systolic and diastolic functions in cardiac hypertrophy.