茯苓复方与有氧运动干预动脉粥样硬化模型大鼠血脂及黏附分子的变化

Effect of Poria compound combined with aerobic exercise on lipid and adhesion molecules in atherosclerosis rats

背景:研究表明有氧运动可以起到抗动脉粥样硬化的作用,而部分中药制剂也对动脉粥样硬化易损斑块形成的相关信号通路具有一定的干预作用。目的:观察有氧运动结合茯苓复方制剂对动脉粥样硬化老龄大鼠血清脂代谢以及黏附分子水平的影响,探索有氧运动和中药对动脉粥样硬化的影响机制以及预防效果。方法:40只SPF级SD老龄大鼠,随机分成对照组、模型组、中药组、联合组4组,每组10只。对照组常规喂养,其余组大鼠腹腔注射维生素D3联合高脂饲料16周喂养建立动脉粥样硬化模型。造模期间中药组和联合组大鼠每天给予20 g/kg复方茯苓制剂灌胃,联合组另外给予1 h/d的无负重游泳运动干预。结果与结论:与对照组相比,模型组大鼠血清三酰甘油、总胆固醇、低密度脂蛋白表达水平及血清黏附分子1和淋巴细胞功能相关分子1浓度上升。与模型组相比,联合组和中药组大鼠血清三酰甘油、总胆固醇、低密度脂蛋白表达水平及血清黏附分子1和淋巴细胞功能相关分子1浓度明显下降,且联合组大鼠血清三酰甘油和低密度脂蛋白表达水平和血清黏附分子1质量浓度低于中药组,而血清高密度脂蛋白表达水平高于中药组。提示有氧运动加复方茯苓制剂联合干预比单纯复方茯苓制剂干预效果好,对动脉粥样硬化的治疗应采取多因素协同的方法以提高治疗的效果。

BACKGROUND： Aerobic exercise has an anti-atherosclerosis effect and some Chinese herbal components have some interventional effects on the atherosclerosis vulnerable plaque. OBJECTIVE： To observe the effects on lipid metabolism and adhesion molecules of atherosclerotic elderly rats after the interventions of Poria compound and aerobics exercise, and explore the mechanism and prevention of hyperlipidemia-induced atherosclerosis. METHODS： Forty Spraque-Dawley elderly rats, of specific pathogen free level, were randomly divided into four groups： control group, model group, medication group, and intervention group, with 10 rats in each group. Rats in the control group were normally fed, while rats in the other groups were intraperitoneally injected with vitamin D3 and high-fat forage for 16 weeks, to establish atherosclerosis model. During the model establishment, rats in the medication and intervention groups were given 20 g/kg Poria compound and Poria compound ＋ 1-hour swimming, respectively. RESULTS AND CONCLUSION： Compared with the control group, total cholesterol, serum triglyceride, low-density lipoprotein expression, intercellular cell adhesion molecule-1 and lymphocyte function association antigen-1 concentrations were increased in the model group. Compared with the model group, these indexes were obviously increased in the medication and intervention groups. The serum triglyceride and low-density lipoprotein expression in the intervention group were lower than those in the medication group, while the serum high-density lipoprotein expression was higher than the medication group. Experimental findings indicate that, the combined interventions of Poria compound and aerobics exercise is better than Poria compound alone, and multi-factor treatment of atherosclerosis should be taken to enhance the efficacy of collaborative approach.