壳寡糖对扩展青霉菌体生长和毒素分泌量的影响

Effects of Oligochitosan on Growth of Penicillium expansum and Production of Patulin

为了更加全面评价壳寡糖作为抑菌剂对病原菌生长和毒素合成之间的内在相关性,采用液体培养的方式,通过静止培养和摇床培养实验,探究了壳寡糖在不同温度培养条件下对扩展青霉菌体生长和展青霉素分泌的影响。结果表明,静止培养中5,10 g/L壳寡糖可明显促进菌体生长,但抑制了毒素的分泌;静止培养和摇床培养实验中,相同浓度的壳寡糖在4,16,25℃培养条件下对菌体生长的影响不同,但是均抑制了毒素分泌;不同温度培养条件下,扩展青霉单位菌体产毒量的大小按培养温度排序为16,4,25℃。因此将壳寡糖用于果蔬采后病害控制时,需要注意贮藏的温度,同时需要考虑其对毒素分泌的影响,以期达到抑菌效果和食用安全性。该研究可为壳寡糖在果蔬采后病害控制中的应用提供参考。

In order to evaluate the antifungal activity of oligochitosan on pathogen growth and mycotoxin production in a comprehensive way, effects of oligochitosan on the growth of Penicillium expansum and the production of patulin were investigated at different temperatures by static and shaking cultivation cultured in liquid medium. The results showed that oligochitosan with concentration of 5, 10 g/L had the obvious promoting effect on the growth of Penicillium expansum, but the inhibitory effect on the production of patulino The same concentration oligochitosan inhibited the production of patulin, but had different effects on the growth of Penicillium expansum at 4, 16 ℃, and 25 ℃. The highest and lowest ratios of patulin and P. expansum （mg：g） were obtained at 16, 25 ℃. This study indicated that application of oligochi- tosan for postharvest disease should take into account the effect of storage temperature and the production of patulin in order to obtain the antifungal effect and food safety.