葡萄籽原花青素对营养肥胖模型大鼠肠道菌群的影响

Effect of Grape Seed Proanthocyanidins on Gut Microbiota of Diet-Induced-Obesity Rats

以高脂膳食喂养大鼠,复制营养肥胖模型,然后灌胃原花青素,采用变性梯度凝胶电泳,实时荧光定量等不依赖微生物培养的手段和多元统计分析方法,研究葡萄籽原花青素对营养肥胖模型大鼠肠道菌群生态的影响。结果表明,低剂量(100 mg/(kg·体重·d))原花青素可以显著抑制大鼠肥胖,处理后大鼠肠道菌群结构与模型组分离;葡萄籽原花青素显著降低了肥胖大鼠肠道菌群中厚壁菌门的含量,提高了拟杆菌门的含量,显著降低了厚壁菌门与拟杆菌门比值;实时荧光定量检测显示,原花青素可以促进拟杆菌增殖,抑制柔嫩梭菌增殖,初步揭示出葡萄籽原花青素可能具有调节肠道菌群的功能;原花青素作用的关键微生物种属为Blautia,Bacteroides,Lactobacillus,Anaerostipes,Clostridium,Anaerofilum。

Wistar rats were fed with high fat diet to induce the diet-induced obesity model （ DIO）, and then gavaged with grape seed proanthocyanidins （GSP） of different doses. Culture-independent methods such as denaturing gradient gel electrophoresis （DGGE） and real-time fluorescent quantitative polymerase chain reaction （real-time PCR） were applied, coupled with multivariate analysis, to explore the effect of GSP on the microbiota of DIO rats. Results showed that GSP treatment （100 mg/（kg.bw-d） ） could in- hibit high-fat induced obesity, and its gut microbiota profile was separated from that of the model group. Results also suggested that GSP-treated groups had the lower Firmicutes content and higher Bacteroidetes content and the ratio of Firmicutes to Bacteroidetes increased in this group. Real-time-PCR results indicated that GSP promoted the proliferation of Bacteriodes spp. and inhibited that of Clostridium leptum. The key genera the GSP might have an effect on were as follows： Blautia, Bacteroides, Lactobacillus, Anaero- stipes, Clostridium, Anaerofilurn.