二苯乙烯苷和三七总皂苷配伍对Aβ_(25-35)致PC12细胞损伤影响的研究

Study of Compatibility of TSG and PNS on PC12 Cell Injury Induced by Aβ_(25-35)

目的探讨二苯乙烯苷(TSG)和三七总皂苷(PNS)配伍对Aβ25-35诱导的PC12细胞损伤的影响。方法 PC12细胞采用Aβ25-35诱导建立阿尔茨海默病的损伤模型,被随机分成空白组、模型组、多奈哌齐组(10μmol/L)、TSG(100 mmol/L)组、PNS组(50 mg/L)、TSG-PNS配伍组(TSG100 mmol/L+PNS50 mg/L)。取细胞上清液测各组的乙酰胆碱酯酶(Ach E)及超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量。结果与模型组比较,TSG组、PNS组、TSG-PNS配伍组可降低Ach E活力、MDA含量及提高SOD活力(P<0.05)。且TSG-PNS配伍组的效应比TSG或PNS单用组效应更好(P<0.01)。结论 TSG和PNS配伍对Aβ25-35诱导的PC12细胞损伤具有明显的保护作用,其作用机制可能与胆碱能系统及氧化应激有关。

Objective To investigate the effect of compatibility of TSG and PNS on PC12 cell injury induced by Aβ25-35.Methods PC12 cell induced by Aβ25-35 was established Alzheimer＇s disease damage models, and PC12 cells were randomly divided into blank group, model group, Donay Patsy group（10 μmol/L）, TSG group（100 mmol/L）, PNS group（50 mg/L）, TSG-PNS group（TSG 100 mmol/L ＋ PNS 50 mg/L）.The acetylcholinesterase（Ach E）, catalase（SOD） activity and malondialdehyde（MDA） content in supernatant were measured. Results Compared with the model group, the TSG group, PNS group and TSG-PNS group can reduce the vigor of Ach E and the content of MDA, and increase the activity of SOD（P〈0.05）. Moreover, the effect of TSG-PNS group was better than only using TSG or PNS（P 0.01）. Conclusion The compatibility of TSG and PNS exhibited a significant protective effect on PC12 cell injury induced by Aβ25-35 and its mechanism may be related to the cholinergic system and oxidative stress.