枸地氯雷他定注射液对大鼠过敏性鼻炎的影响

Effect of Desloratadine Citrate Disodium Injections on Ovalbumin-induced Allergic Rhinitis in Rats

目的:研究枸地氯雷他定注射液对卵白蛋白(OVA)致大鼠过敏性鼻炎的鼻部症状及鼻腔灌洗液PGD2、LTC4含量和炎症细胞浸润的影响。方法:采用OVA建立大鼠过敏性鼻炎模型,将造模成功的SD雄性大鼠随机分成5组:模型组、枸地氯雷他定注射液(简称枸地)低、中、高剂量组,阳性药马来酸氯苯那敏注射液(扑尔敏)组。另随机取正常大鼠设正常组。造模第22天,模型组和正常组静注生理盐水;枸地低、中、高剂量组分别静注0.3、0.6、1.2 mg·kg-1;扑尔敏组静注0.6 mg·kg-1。各组每天一次用药,连续5天,同时隔天以OVA滴鼻以维持过敏状态。于最后一次给药半小时后OVA滴鼻激发观察30 min内大鼠抓鼻、喷嚏及流涕情况,采取积分法记录上述症状程度;2 h后收集大鼠鼻腔灌洗液(NLF)测定PGD2、LTC4含量及炎症细胞数量。结果与结论:枸地组可显著改善过敏性鼻炎大鼠鼻部症状,且能减少NLF中炎症介质PGD2、LTC4含量及炎症细胞数量,改善过敏性鼻炎大鼠鼻部症状,同时也能抑制白细胞浸润,减轻晚期慢性炎症反应。

Objective: To study the effect of desloratadine citrate disodium injections (DLC) on ovalbumin-induced allergic rhinitis in rats, including the rhinitis symptoms, PGD2 and LTC4 levels and inflammatory cell infiltration in nasal lavage fluid (NLF). Methods: Ovalbumin-induced allergic rhinitis male SD rats were randomly divided in five groups: model group, DLC low, medium and high dose groups, and chlorphenamine maleate injectlon (CHM) group as positive group. In addition, the normal rats were also recorded. From the 22nd day of being modeled, rats of the model and normal groups were given iv saline, the DLC low, medium and high dose groups were given DLC at 0.3, 0.6 and 1.2 mg·kg-1, CHM group were given CHM at 0.6 mg·kg-1, all the iv treatments were once a day for five days. Rats were topically sensitized by instilling ovalbumin solution into the bilateral nasal cavities on alternate days. Symptoms of rhinitis were observed continuously for 30 min at the last time for administration and topically sensitization, taking the scoring method to record the symptoms of rhinitis. Two hours after the intranasal challenge, NLF was collected, PGD2 and LTC4 levels and inflammatory cellular infiltration were evaluated, respectively. Results and Conclusion: DLC significantly attenuated ovalbumin-induced rhinitis symptoms and caused a significant reduction in PGD2 and LTC4 levels and inflammatory cellular infiltration.