摘要
基于生物信息学和实验预测,克隆了柑橘抗寒资源枳ptf-miR168a成熟序列和前体序列,并预测了其靶基因。结果表明:ptf-miR168a在植物中高度保守,靶基因是NAC(UCU2)和锌指结构基因(FEZ)家族同源基因;同时,构建了ptf-miR168a前体的超表达载体并在烟草中进行遗传转化,得到了18个转基因系,为进一步鉴定ptf-miR168a在低温胁迫应答中的调控作用奠定基础。
miRNAs(microRNAs)are a type of conserved,endogenous and non-coding small RNAs in plants,and regulate their target genes at post-transcriptional level,which plays important roles in plant.Poncirus trifoliatais a cold-tolerant citrus relative and is often used as rootstock in citrus industry.The mature sequence and precursor sequence were identified in this study.The results showed that ptfmiR168 asequence was highly conserved in plants.The target genes of ptf-miR168 awere analyzed and predicted to be NAC transcription factors and zinc finger genes.The over-expression vector was constructed and transformed to tobacco.A total of 18 transgenic lines were obtained.It will provide valuable materials for further studying the regulatory role of ptf-miR168 ain response to cold stress.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2015年第6期14-20,共7页
Journal of Huazhong Agricultural University
基金
农业部农业科研杰出人才计划项目
关键词
miR168
枳
抗寒性
靶基因
克隆
遗传转化
低温胁迫
miR168
Poncirus trifoliata(L.)Raf.
cold tolerance
target gene
cloning
genetic transformation
low-temperature stress