摘要
通过生物信息学分析,在链霉菌SH-62的基因组中发现一个Ⅱ型聚酮合酶基因簇(ents),与已报道的来源于Streptomyces maritimus的肠菌素生物合成基因簇具有高度的相似性。通过构建和筛选链霉菌SH-62的基因组细菌人工染色体(BAC)文库,克隆得到完整的ents基因簇。将ents基因簇导入白色链霉菌中进行异源表达,HPLC和LC-MS分析结果表明该基因簇负责肠菌素的生物合成,从而证实了ents基因簇的生物学功能。
Bioinformatics analysis of Streptomyces sp.SH-62 genome sequence showed that there was a typeⅡpolyketide synthase gene cluster(ents)having high homology with the enterocin biosynthetic gene cluster in Streptomyces maritimus.The entire ents cluster was cloned by constructing and screening the genomic bacterial artificial chromosome(BAC)library of Streptomyces sp.SH-62.The ents cluster was introduced into Streptomyces albus for heterologous expression.The results of HPLC and LC-MS analysis confirmed that the ents cluster is responsible for the enterocin biosynthesis in Streptomyces sp.SH-62.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2015年第6期55-60,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31270136)
教育部留学回国人员科研启动基金项目([2009]1590)
教育部新世纪人才支持计划项目(NCET-08-0779)
关键词
链霉菌SHG62
肠菌素
细菌人工染色体
白色链霉菌
异源表达
Streptomyces sp.SH-62
enterocin
bacterial artificial chromosome(BAC)
Streptomyces albus
heterologous expression
