CaNAα基因在清远麻鸡和隐性白羽鸡不同表型肌肉中的表达

Expression profile of CaNAα gene in phenotypically distinct muscles of two chicken breeds with different growth rate

为探讨CaNAα在生长速度差异较大的2个品种鸡生长发育早期不同表型肌肉中的表达规律,利用实时荧光定量PCR技术检测清远麻鸡(慢生型)和隐性白羽鸡(速生型)3种表型肌肉(腓肠肌外侧头、比目鱼肌和趾长伸肌)CaNAαmRNA在0、1、3、5、7、9周龄时的表达情况。结果发现:除清远麻鸡比目鱼肌外,2个鸡品种中其他表型肌肉均呈现先上升后下降然后再上升的趋势;2个鸡品种趾长伸肌的表达量均在1周龄时最高,清远麻鸡7周龄时降至最低,而隐性白羽鸡5周龄时降至最低;腓肠肌外侧头和比目鱼肌CaNAαmRNA的表达峰值在清远麻鸡中出现在3周龄,而在隐性白羽鸡中则出现在9周龄;总体而言,CaNAαmRNA在比目鱼肌中的表达量要低于其在趾长伸肌和腓肠肌外侧头中的表达量;品种间比较发现,CaNAαmRNA在清远麻鸡3种表型肌肉的表达量在0和3周龄时均高于隐性白羽鸡,而在9周龄时则均低于隐性白羽鸡,3周龄时2个品种鸡差异倍数最高。研究结果提示,鸡肌肉CaNAαmRNA表达发育模式具有品种、年龄和表型特异性,并可能与鸡肉品质存在关联。

To explore the expression profile of the CaNAαgene in phenotypically distinct muscles during early growth development,quantitative real-time PCR was applied to detect the CaNAα mRNA in the lateral gastrocnemius,soleus and extensor digitorum longus muscles at different weeks of ages(0,1,3,5,7,9w)in Recessive White chicken(fast-growing chicken breed)and Qingyuan partridge chicken(slow-growing chicken breed).The results showed that except for the soleus muscle in Qingyuan partridge chicken,the CaNAα mRNA of other muscles in the two chicken breeds was firstly increased,then decreased and finally increased again.The highest CaNAα mRNA level in the entensor digitorum longus in both chicken breeds peaked at 1w,and the lowest level appeared at 7win Qingyuan partridge chicken while at 5 win Recessive White chicken.The CaNAα mRNA in the lateral gastrocnemius and soleus muscles in Qingyuan partridge chicken peaked at 3w,while at 9win Recessive White chicken.Overall,the CaNAαexpression in the soleus was lower than that in the extensor digitorum longus and lateral gastrocnemius muscles.The CaNAα mRNA of the three phenotypically distinct muscles in Qingyuan partridge chicken was higher at 0and 3w,but lower at 9wthan that of Recessive White chicken,with extremely significantly difference appeared at 3w(P<0.01).Overall,the CaNAαgene in the chicken muscle showed specific developmental patterns,which may be related to the breed,age,phenotype,and the chicken meat quality as well.