“肤敏”膏对大鼠PCA模型T淋巴细胞及其亚群的影响

The Influence of FuMin Ointment on T Lymphocytes and Its Subsets of PCA Rat Model lymphocyte

目的:研究"肤敏"膏对大鼠被动皮肤过敏反应(PCA)模型T淋巴细胞及其亚群的影响,探讨其抗皮肤过敏作用机理。方法:用卵白蛋白建立大鼠PCA模型,采用免疫荧光流式细胞技术检测PCA大鼠外周血CD3+T、CD4+T和CD8+T细胞表达率及"肤敏"膏对3种细胞的影响作用。结果:与模型组比较,"肤敏"膏3剂量组CD3+T和CD4+T细胞表达率均值较模型组明显升高(P<0.05)。结论:"肤敏"膏的作用机理可能与其调节CD3+T、CD4+T的数量紊乱有关,从而间接的干预Th1/Th2细胞分化及平衡,在过敏反应早期调节细胞免疫平衡以阻断病变进程。

Objective： To explore the mechanism of Fu Min ointment fighting against skin allergic reaction by studying its effects on T lymphotes and its subsets of passive cutaneous anaphylaxis（PCA） rat model. Methods： PCA rat model was established with ovalbumin, expression rates of CD3＋T, CD4＋T and CD8＋T in peripheral blood of PCA rats were detected by immunofluorescent flow cytometry and the effects of Fu Min ointment on three kinds of cells. Results： Compared with the model group, three groups of Fu Min ointment were higher than the model group in CD3＋T,CD4＋T and CD8＋T cellular expression rates average values obviously（P0.05）. Conclusion： The mechanism of Fu Min ointment might be related to its function of regulating CD3＋T and CD4＋T quantity disorder indirectly, therefore, to intervene Th1/Th2 cells differentiation and balance, regulating cellular immunity balance in allergic reaction in order to cease pathological progress.